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SAB4200715

Anti-β-tubulin antibody, Mouse monoclonal

Name: Anti-β-tubulin antibody, Mouse monoclonal
Brand: SIGMA

clone 2-28-33, purified from hybridoma cell culture

Synonyme(s) :

Anti-TUBB1

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About This Item

Code UNSPSC :

12352203

Nomenclature NACRES :

NA.41

Source biologique

mouse

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

2-28-33, monoclonal

Forme

buffered aqueous solution

Poids mol.

~50 kDa

Espèces réactives

dog, chicken, rat, bovine, monkey, human, mouse

Concentration

~1.0 mg/mL

Technique(s)

immunoblotting: 0.5 μg/mL using mouse embryo fibroblast NIH-3T3 cells extract
immunofluorescence: 1-2 μg/mL using human foreskin fibroblast HS68 cells
immunohistochemistry: suitable

Isotype

IgG1

Numéro d'accès UniProt

P07437

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TUBB(203068)
mouse ... Tubb3(22152)

Description générale

Anti-β-tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 2-28-33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin also known as TUBB is the major building block of microtubules. Microtubules assemble from heterodimers of α-tubulin and β-tubulin; both have a molecular weight of ~ 50 kDa and share considerable homology. For β-tubulin, five evolutionarily conserved isotypes have been identified, which are regularly utilized in the same cell types of different species.

Immunogène

Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Application

Monoclonal Anti-β-tubulin has been used:

in immunoblotting
in immunofluorescence
in immunohistochemistry

Actions biochimiques/physiologiques

Monoclonal Anti-β-tubulin specifically recognizes the two major β-tubulin isotypes and one of the minor β-tubulin isotypes of the free-living soil nematode Caenorhabditis elegans. It also binds to Sarkosyl resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, neuronal axons of the larva of Ciona intestinalis (ascidian), microtubules in root tip cells in Arabidopsis thaliana and to human, mouse, rat, monkey, bovine, dog and chicken β-tubulin. The different β-tubulin isotypes are suggested to differ from each other in their ability to polymerize into microtubules.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Icariin Attenuates High Glucose-Induced Apoptosis, Oxidative Stress, and Inflammation in Human Umbilical Venous Endothelial Cells

Sun S, et al.

Planta Medica, 85(06), 473-482 (2019)

Down-regulation of Cx43 expression on PIH-HUVEC cells attenuates monocyte-endothelial adhesion

Li X, et al.

Thrombosis Research, 179, 104-113 (2019)

Structure of the caudal neural tube in an ascidian larva: vestiges of its possible evolutionary origin from a ciliated band.

R J Crowther et al.

Journal of neurobiology, 23(3), 280-292 (1992-04-01)

Ultrastructural analysis and differential immunocytochemical staining with two antitubulin monoclonal antibodies were used to reexamine the organization and development of the neural tube in the larva of an ascidian, Ciona intestinalis, in appraisal of a theory that the dorsal tubular

Anti-tubulin monoclonal antibodies that bind to specific neurons in Caenorhabditis elegans.

S S Siddiqui et al.

The Journal of neuroscience : the official journal of the Society for Neuroscience, 9(8), 2963-2972 (1989-08-01)

We have identified 2 anti-tubulin monoclonal antibodies that bind to 2 different subpopulations of identified neurons in the simple nervous system of the nematode Caenorhabditis elegans. The antibodies also recognize specific tubulin isotypes from C. elegans that were separated by

Expression Analyses of Rac3, a Rho Family Small GTPase, during Mouse Brain Development.

Masashi Nishikawa et al.

Developmental neuroscience, 44(1), 49-58 (2021-11-29)

Rac3 is a member of Rho family small GTPases which regulate cellular signaling and cytoskeletal dynamics. The RAC3 gene abnormalities have been shown to cause neurodevelopmental disorders with structural brain anomalies, including polymicrogyria/dysgyria, callosal abnormalities, brainstem anomalies, and cerebellar dysplasia.

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