SAB4200654
Monoclonal Anti-CHD1 antibody produced in rat
clone 2F11H5, purified from hybridoma cell culture
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rat
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
2F11H5, monoclonal
Forme
buffered aqueous solution
Espèces réactives
human
Concentration
~1 mg/mL
Technique(s)
flow cytometry: 5-10 μg/test using using HeLa cells
immunofluorescence: 20 μg/mL using using human HeLa cells.
Isotype
IgG2a
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... CHD1(1105)
Description générale
Monoclonal Anti-CHD1 (rat IgG2a isotype) is derived from the hybridoma 2F11H5 produced by the fusion of mouse myeloma cells and lymph node cells from rat immunized with a synthetic peptide. Chromodomain-helicase-DNA-binding protein 1 (CHD1) is an ATP-dependent chromatin-remodeling factor expressed majorly in cerebellum and basal ganglia. CHD1 gene is mapped to human chromosome 5q15-q21.1. CHD1 comprises two chromodomains.
Spécificité
Monoclonal Anti-CHD1 recognizes human CHD1.
Immunogène
synthetic peptide corresponding to a sequence at the C-terminal region of human CHD1.
Application
Monoclonal Anti-CHD1 antibody produced in rat may be used in:
- immunofluorescence
- flow cytometry
- immunoblotting
- immunohistochemistry
Actions biochimiques/physiologiques
Chromodomain-helicase-DNA-binding protein 1 (CHD1) interacts with the trimethylated lysine residues of histones and alters access of the transcriptional apparatus to its chromosomal DNA template. CHD1 is required for maintenance of open chromatin and pluripotency in embryonic stem cells. Missense mutation in the CHD1 gene is implicated in the Pilarowski-Bjornsson syndrome as well as in speech delay and facial dismorphism.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Stockage et stabilité
For continuous use, store at 2–8 °C for up to one month. For extended storage,freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Missense variants in the chromatin remodeler CHD1 are associated with neurodevelopmental disability
Pilarowski GO, et al.
Journal of medical Genetics, 55(8), 561-566 (2018)
Di Zhao et al.
Cancer discovery, 10(9), 1374-1387 (2020-05-10)
Genetic inactivation of PTEN is common in prostate cancer and correlates with poorer prognosis. We previously identified CHD1 as an essential gene in PTEN-deficient cancer cells. Here, we sought definitive in vivo genetic evidence for, and mechanistic understanding of, the
Saori Yoshimura et al.
Hybridoma (2005), 29(1), 63-66 (2010-03-05)
CHD5 (chromodomain/helicase/DNA-binding protein 5) is a member of the CHD subfamily of chromatin remodeling Swi/Snf proteins, and has been recently identified as a tumor suppressor in a diverse range of human cancers. We report here on the establishment of a
Glenn Hauk et al.
Current opinion in structural biology, 21(6), 719-727 (2011-10-15)
This review focuses on recent structural insights into regulation and nucleic acid binding of Superfamily 2 (SF2)-type helicases as they relate to chromatin remodelers. We review structural features of the Chd1 chromatin remodeler regarding regulation of the ATPase motor, and
Lena Ho et al.
Nature, 463(7280), 474-484 (2010-01-30)
New methods for the genome-wide analysis of chromatin are providing insight into its roles in development and their underlying mechanisms. Current studies indicate that chromatin is dynamic, with its structure and its histone modifications undergoing global changes during transitions in
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