SAB4200580

Anti-Msi-1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonyme(s) : AntiMSI1, AntiMSI1H, AntiMUSASHI 1, Antim-Msi-1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~39 kDa
• Espèces réactives: human
• Concentration: ~1.0 mg/mL
• Technique(s): immunoblotting: 1-2 μg/mL using Lysates of HEK-293T cells overexpressing human Msi-1.; immunoprecipitation (IP): 5-10 μg using HEK-293T cells overexpressing human Msi-1.; indirect immunofluorescence: 5-10 μg/mL using HeLa cells.
• Numéro d'accès UniProt: O43347
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MSI1(4440) ; rat ... Msi1(259272)

Description

Description générale

Musashi-1 (Msi-1) is highly expressed in neural stem/progenitor cells (NS/PCs). MSI1 gene is mapped to human chromosome 12q24.

Immunogène

synthetic peptide corresponding to an internal sequence of human Msi-1, conjugated to KLH. The corresponding sequence is identical in rat and highly conserved (94% identity) in mouse Msi-1.

Application

Anti-Msi-1 antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Actions biochimiques/physiologiques

Musashi-1 (Msi-1) is a neural RNA-binding protein that regulate translation of target mRNAs. It is associated with maintenance and asymmetric cell division of neural stem cells. Msi-1 binds to the 3′-untranslated region (UTR) of its target mRNAs in neural stem/progenitor cells (NS/PCs), repressing their translation, and interfering with NS/PC differentiation thus maintaining NP/PCs in the undifferentiated state. Loss of Msi-1 function disrupts the balance between germ-line stem cell renewal and differentiation, causing premature differentiation. Msi-1 has been shown to regulate the Notch and Wnt signaling pathway. Upregulation of Msi-1 augments Notch signaling, via translational repression of the Notch signaling antagonist m-Numb, thereby inhibiting the differentiation of neural stem cells into neurons. Msi-1 is also expressed in the nucleus during the early neural differentiation of mouse embryonic stem cells (ESCs) and found to act synergistically with Lin28 as a novel cofactor for the blockade of let-7 family miRNA biogenesis. Expression of Msi1- has been detected in several human cancers including brain tumors, suggesting its role in oncogenic development.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable