SAB4200485

Anti-acetyl-Histone H3 (Ac-Lys²⁷) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonyme(s) : Anti-H3.4, Anti-H3/g, Anti-H3FT, Anti-H3K27ac, Anti-H3t, Anti-HIST3H3, Anti-Hist1h2ai, Anti-Hist1h2ail, Anti-Hist1h3a, Anti-Hist1h3g, Anti-Hist1h3h, Anti-Hist1h3i
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~15 kDa
• Espèces réactives: human, rat, mouse
• Concentration: ~1.0 mg/mL
• Technique(s): immunohistochemistry: 10-20 μg/mL; indirect immunofluorescence: 2.5-5.0 μg/mL using HeLa cells; western blot: 1-2 μg/mL using extracts of NIH3T3 cells
• Numéro d'accès UniProt: Q16695
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: acetylation (Lys27)
• Informations sur le gène: human ... HIST3H3(8290)

Description

Description générale

The HIST3H3 gene is mapped on the human chromosome at 1q42.13. Histones are proteins that form the nucleosome along with DNA. A nucleosome consists of core histone octamers made of two heterodimers of H2A and H2B along with two heterodimers of H3 and H4. This octamer is bound by the DNA and H1 protein.

Spécificité

Anti-acetyl-Histone H3 (Ac-Lys27) specifically recognizes human and mouse (Ac-Lys27) Histone H3 (H3K27Ac).

Immunogène

synthetic peptide containing acetylated Lys27 of human histone H3, conjugated to KLH. The corresponding sequence is identical in many species including rat and mouse histone H3

Application

Anti-acetyl-Histone H3 (Ac-Lys27) antibody produced in rabbit may be used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

Actions biochimiques/physiologiques

Histones are subjected to extensive covalent modifications, including phosphorylation, methylation, acetylation and ubiquitination. Active chromatin is highly correlated with the hyperacetylation of histone H3 at the N-terminal tail. Histone H3 can be reversibly acetylated at Lys residues 9, 14, 18, 23, 27, 36 and 56 by a family of enzymes known as histone acetyltransferases (HATs). Histone H3 can be modified at Lys27 either by methylation or acetylation. Histones are thought to play an important role in development and cancer.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable