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SAB4200369

Sigma-Aldrich

Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse

clone R2.5254.2, purified from hybridoma cell culture

Synonyme(s) :

Anti-CD112, Anti-HVEB, Anti-PRR2, Anti-PVRL2, Anti-PVRR2, Anti-Poliovirus receptor-related 2 (herpesvirus entry mediator B)

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

R2.5254.2, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~72 kDa

Espèces réactives

rat, human, monkey, mouse

Concentration

~1.0 mg/mL

Technique(s)

flow cytometry: 5-10 μg/test using using 1x106 MDA-MB-231 cells
immunocytochemistry: 2.5-5 μg/mL using using MDA-MB-231 cells
indirect ELISA: suitable
western blot: suitable

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

Description générale

Monoclonal Anti-Nectin-2/PVRL2 (mouse IgG1 isotype) is derived from the hybridoma R2.525.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with 3T3 cells expressing human nectin-2. Nectin-2 is found to be widely expressed in human tissues, including hematopoietic cells.
Nectin-2, also known as poliovirus receptor related protein 2 gene (PRR2), is encoded by the gene mapped to human chromosome 19q13.2. The encoded protein belongs to the nectin protein family and is characterized with an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain.

Spécificité

The antibody has been reported to block cell adhesion and herpes virus entry.

Immunogène

3T3 cells expressing human Nectin-2.

Application

Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry
  • flow cytometry

Actions biochimiques/physiologiques

Nectins act as key elements of adherens junctions and facilitate their formation. Nectin-2 possesses anti-viral property against herpes simplex virus (HSV)-2 infection. In mice, loss of nectin-2 affects sperm production and male-specific infertility. Aberrations in the gene has been associated with the pathogenesis of peripheral T-cell lymphoma (PTCL). Serum levels of nectin-2 can be used as a diagnostic and prognostic marker for colorectal cancer (CRC).

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Elevated Nectin-2 expression is involved in esophageal squamous cell carcinoma by promoting cell migration and invasion
Li M, et al.
Oncology Letters, 15(4) (2018)
Serum nectin-2 levels are diagnostic and prognostic in patients with colorectal carcinoma.
Karabulut M
Clinical And Translational Oncology, 18, 160-171 (2016)
Sequencing of 42kb of the APO E-C2 gene cluster reveals a new gene: PEREC1.
Dna Sequence, 9, 89-100 (1998)
PVRL2 is translocated to the TRA@ locus in t(14;19)(q11;q13)-positive peripheral T-cell lymphomas.
Almire C
Genes Chromosomes Cancer, 46, 1011-1018 (2007)
Viral entry mechanisms: cellular and viral mediators of herpes simplex virus entry.
Akhtar J and Shukla D
FEBS Journal, 276, 7228-7236 (2009)

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