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Key Documents

SAB4200344

Sigma-Aldrich

Anti-SMYD3 antibody, Mouse monoclonal

clone SMYD3-2, purified from hybridoma cell culture

Synonyme(s) :

Anti-KMT3E, Anti-SET and MYND domain containing 3, Anti-ZMYND1, Anti-Zinc finger MYND domain-containing protein 1, Anti-Zinc finger protein, subfamily 3A (MYND domain containing), 1

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

SMYD3-2, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~43 kDa

Espèces réactives

rat, mouse, human

Concentration

~1.0 mg/mL

Technique(s)

western blot: 5-10 μg/mL using whole extracts of HeLa cells

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... SMYD3(64754)
mouse ... Smyd3(69726)
rat ... Smyd3(498295)

Description générale

Monoclonal Anti-SMYD3 (mouse IgG1 isotype) is derived from the hybridoma SMYD3-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein corresponding to a fragment of human SMYD3. SMYD3 belongs to the SMYD family of chromatin regulators. It is a histone methyltransferase, that plays a role in transcriptional regulation as a member of an RNA polymerase complex. SMYD3 contains a SET domain, which is critical for the methylation of histone H3 at Lys4 and a myeloid, Nervy, and DEAF-1 (MYND)-type zinc-finger domain, which is common to developmental proteins.

Immunogène

Fusion protein corresponding to a fragment of human SMYD3.

Application

Monoclonal Anti-SMYD3 antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

Actions biochimiques/physiologiques

SMYD3 is important in heart and skeletal muscle development. Overexpression of SMYD3 is observed in hepatocellular cancer, colorectal cancer and breast cancer. SMYD3 is essential for estrogen receptor-regulated gene transcription in the estrogen signaling pathway.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells
Hamamoto R, et al.
Nature Cell Biology, 6(8), 731-731 (2004)
Requirement of histone methyltransferase SMYD3 for estrogen receptor-mediated transcription
Kim H, et al.
The Journal of Biological Chemistry, 284(30), 19867-19877 (2009)
Structural insights into the autoinhibition and posttranslational activation of histone methyltransferase SmyD3
Sirinupong N, et al.
Journal of Molecular Biology, 406(1), 149-159 (2011)

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