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Principaux documents

SAB4200203

Sigma-Aldrich

Anti-RAVER1 antibody, Mouse monoclonal

clone RAV1, purified from hybridoma cell culture

Synonyme(s) :

Anti-Ribonucleoprotein PTB-binding 1

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Conjugué:
unconjugated
application:
IP
WB CL
Clone:
RAV1, monoclonal
Espèces réactives:
human, monkey
citations:
3
Technique(s):
immunoprecipitation (IP): suitable
western blot (chemiluminescent): 1.0-2.0 μg/mL using HeLa or HepG2 cells extract

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

RAV1, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~80 kDa

Espèces réactives

human, monkey

Concentration

~1.0 mg/mL

Technique(s)

immunoprecipitation (IP): suitable
western blot (chemiluminescent): 1.0-2.0 μg/mL using HeLa or HepG2 cells extract

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... RAVER1(125950)

Description générale

Monoclonal Anti-RAVER1 (mouse IgG1 isotype) is derived from the hybridoma RAV1 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from mouse immunized with a peptide corresponding to a fragment of human RAVER1. Raver1, also known as ribonucleoprotein PTB-binding 1, is a widely expressed multidomain protein, identified in two-hybrid screens by its ability to interact and colocalize with the cytoskeletal proteins -actinin and vinculin. The protein is composed of three RNA recognition motifs (RRM) and of nuclear localization and nuclear export signals, allowing it to shuttle between the nucleus and the cytoplasm. Raver1 also colocalizes with PTB/hnRNPI.

Application

Monoclonal Anti-RAVER1 antibody produced in mouse has been used in immunoblotting.

Actions biochimiques/physiologiques

RAVER1 (ribonucleoprotein, PTB binding 1) is involved in RNA splicing of microfilament proteins. In skeletal muscle, a translocation of Raver1 from the nucleus to the cytoplasm is correlated with the differentiation of specific microfilament attachment sites. Based on an analysis of Vinculin-Raver1 crystal structure it was suggested that vinculin recruits raver1 and its mRNAs cargo to focal adhesions, promoting localization of the synthesis of adhesion complexes by the translational machinery.

Forme physique

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins
Huttelmaier, et al.
The Journal of Cell Biology, 155, 775-775 (2001)
Raver1 Interactions with Vinculin and RNA Suggest a Feed-Forward Pathway in Directing mRNA to Focal Adhesions
Lee J H, et al.
Structure, 17, 833-842 (2009)
K Meletis et al.
The Journal of cell biology, 155(5), 699-702 (2001-11-29)
Recent studies have shown that cells expressing neuronal antigens can be derived from a bone marrow transplant. A new report lends support to and extends these previous results by presenting compelling morphological evidence for the generation and integration of highly

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