Accéder au contenu
Merck
Toutes les photos(1)

Documents

SAB4200175

Sigma-Aldrich

Anti-Atg101 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonyme(s) :

Anti-Atg13-interacting protein, Anti-C12orf44, Anti-autophagy-related protein 101, Anti-chromosome 12 open reading frame 44

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

~30 kDa

Espèces réactives

human

Concentration

~1.0 mg/mL

Technique(s)

western blot: 0.25-0.5 μg/mL using whole extracts of HEK-293T cells over expressing human Atg101

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... C12orf44(60673)

Description générale

Autophagy101 (Atg101) is located on the human chromosome at 12q13.13.

Application

Anti-Atg101 antibody has been used in
  • immunoblotting
  • immunofluorescence
  • immunopurification

Actions biochimiques/physiologiques

Autophagy101 (Atg101) is a mammalian Autophagy13 (Atg13) binding protein essential for autophagy. Atg13 forms a stable complex with ULK1 (Serine/threonine-protein kinase ULK1) and FIP200 (FAK family kinase-interacting protein of 200 kDa). Atg101 associates with the ULK1-Atg13- FIP200 complex through direct interaction with Atg13. Mammalian target of rapamycin (mTOR) interacts with the ULK1-Atg13-FIP200 complex in a nutrient dependent manner, suggesting that mTOR regulates autophagy through this complex.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells
Hieke N, et al.
Autophagy, 11(9), 1471-1483 (2015)
Molecular Machinery and Genetics of the Autophagy Pathway
Autophagy in health and disease, 16-16 (2012)
Mei Yang et al.
Science advances, 2(9), e1601167-e1601167 (2016-09-13)
The intronic GGGGCC hexanucleotide repeat expansion in chromosome 9 open reading frame 72 (C9ORF72) is a prevalent genetic abnormality identified in both frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). Smith-Magenis syndrome chromosomal region candidate gene 8 (SMCR8) is a
Atg101, a novel mammalian autophagy protein interacting with Atg13
Hosokawa N, et al.
Autophagy, 5(7), 973-979 (2009)
Eleftherios Karanasios et al.
Nature communications, 7, 12420-12420 (2016-08-12)
Autophagosome formation requires sequential translocation of autophagy-specific proteins to membranes enriched in PI3P and connected to the ER. Preceding this, the earliest autophagy-specific structure forming de novo is a small punctum of the ULK1 complex. The provenance of this structure
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique