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SAB4200048

Sigma-Aldrich

Anti-E1B-AP5 antibody, Rat monoclonal

clone EAP4A11, purified from hybridoma cell culture

Synonyme(s) :

Anti-Adenovirus E1B 55-KD protein-associated protein 5, Anti-Heterogeneous nuclear ribonucleoprotein U-like 1, Anti-hnRNPUL1, Anti-hnRPUL1, Monoclonal Anti-E1B-AP5 antibody produced in rat

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rat

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

EAP4A11, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~110 kDa

Espèces réactives

bovine, canine, human, rat, monkey, mouse

Technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.05-0.1 μg/mL using HT-29 cell extracts

Isotype

IgG2b

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

Catégories apparentées

Description générale

Monoclonal Anti-E1B-AP5 (rat IgG2b isotype) is derived from the hybridoma EAP4A11 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with full length human E1B-AP5 fusion protein. E1B-55K-associated protein 5 (E1B-AP5), also known as heterogeneous nuclear ribonucleoprotein U-like 1(hnRNPUL1), is a nuclear RNA-binding protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) family.
The E1B-AP5 gene is located on the human chromosome at 19q13.2.

Spécificité

Monoclonal Anti-E1B-AP5 recognizes human, monkey, bovine, dog, mouse and rat E1B-AP5.

Immunogène

full length human E1B-AP5 fusion protein.

Application

Monoclonal Anti-E1B-AP5 antibody produced in rat may be used in:
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry

Actions biochimiques/physiologiques

E1B-55K-associated protein 5 (E1B-AP5) plays a vital role in ATR signaling. In addition, it may play an important role in nucleocytoplasmic RNA transport, and its function is modulated by E1B55kDa in adenovirus-infected cells. Interestingly, this protein was found to be associated with bromodomain-containing protein, BRD7 in a complex that links chromatin events with mRNA processing at the level of transcriptional regulation. It also activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation.

Forme physique

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Regulation of transcription by the heterogeneous nuclear ribonucleoprotein E1B-AP5 is mediated by complex formation with the novel bromodomain-containing protein BRD7.
Kzhyshkowska J, et al.
The Biochemical Journal, 371, 385-385 (2003)
Regulation of DNA-End Resection by hnRNPU-like Proteins Promotes DNA Double-Strand Break Signaling and Repair
Polo SE, et al.
Molecular Cell, 45, 505-516 (2012)
The interaction of the hnRNP family member E1B-AP5 with p53
Barral PM, et al.
Febs Letters, 579, 2752-2758 (2005)
S Gabler et al.
Journal of virology, 72(10), 7960-7971 (1998-09-12)
The adenovirus type 5 (Ad5) early 1B 55-kDa protein (E1B-55kDa) is a multifunctional phosphoprotein that regulates viral DNA replication and nucleocytoplasmic RNA transport in lytically infected cells. In addition, E1B-55kDa provides functions required for complete oncogenic transformation of rodent cells
J Kzhyshkowska et al.
The Biochemical journal, 358(Pt 2), 305-314 (2001-08-22)
The heterogeneous nuclear ribonucleoprotein (hnRNP) family includes predominantly nuclear proteins acting at different stages of mRNA metabolism. A characteristic feature of hnRNPs is to undergo post-translational asymmetric arginine methylation catalysed by different type 1 protein arginine methyltransferases (PRMTs). A novel

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