SAB4200024

Anti-Notch 1 antibody, Rat monoclonal

clone ICN 5B5, purified from hybridoma cell culture

• Synonyme(s) : Anti-Notch homolog 1, translocation-associated (Drosophila), Anti-TAN1, hN1, neurogenic locus notch homolog protein 1, Anti-Translocation-associated notch protein, Monoclonal Anti-Notch 1 antibody produced in rat
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Conjugué: unconjugated
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: ICN 5B5, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~120 kDa
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μL
• Concentration: ~1.0 mg/mL
• Technique(s): western blot: 2-4 μg/mL using whole extract of human Jurkat cells and developing with a sensitive film
• Isotype: IgG2b
• Numéro d'accès UniProt: P46531
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... NOTCH1(4851)

Description

Description générale

Monoclonal Anti-Notch 1 (rat IgG2b isotype) is derived from the hybridoma ICN 5B5 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a recombinant fusion protein corresponding to a fragment of human Notch 1 fusion protein. Notch receptor 1 (Notch1) gene is located on human chromosome 9q34.

Application

Monoclonal Anti-Notch 1 antibody produced in rat has been used in immunoblotting.

Actions biochimiques/physiologiques

In chronic obstructive pulmonary disease, notch receptor 1 (Notch1) helps to modulate endothelial apoptosis via the extracellular signal-regulated kinase (ERK) pathway. Overexpression of Notch1 helps to guard endothelial cell from apoptosis. Knockdown of Notch1 results in the enhancement of apoptotic cells. Notch1 serves as a tumor suppressor gene in mammalian epidermis and is a determinant of keratinocyte differentiation. Notch signaling is believed to participate in several human neoplasms including T-cell acute lymphoblastic leukemia.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable