SAB3501192

Anti-TRIM5 delta antibody produced in rabbit

affinity isolated antibody

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: liquid
• Espèces réactives: human
• Concentration: 1 mg/mL
• Technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_149084
• Numéro d'accès UniProt: Q9C035
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TRIM5(85363)

Description

Description générale

TRIM5 delta Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has five alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. The TRIM5δ isoform has been shown to colocalize with the topoisomerase I-interacting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5δ also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.

Immunogène

TRIM5 delta antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human TRIM5 delta.The immunogen is located within the last 50 amino acids of TRIM5 delta.

Application

TRIM5 delta antibody can be used for detection of TRIM5 delta by Western blot at 2 μg/mL. A band at approximately 25 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Forme physique

Supplied in PBS containing 0.02% sodium azide.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable