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Key Documents

SAB2702215

Sigma-Aldrich

Monoclonal Anti-Strep Tag antibody produced in mouse

clone GT517, affinity isolated antibody

Synonyme(s) :

Mouse Anti-Strep Tag, Strep Tag Detection Antibody

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

mouse

Niveau de qualité

100

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

GT517, monoclonal

Forme

buffered aqueous solution

Concentration

1mg/mL

Technique(s)

indirect immunofluorescence: suitable
western blot: 1000-10000

Isotype

IgG1

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

The strep-tag/strep-tagII (improved version) is made up of eight amino acid sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys).

Immunogène

The immunogen used to generate this antibody corresponds to Strep Tag

Application

Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Actions biochimiques/physiologiques

The strep-tag/strep-tagII (improved version) has the ability to bind to any protein or peptide of interest.

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Autres remarques

Purification: Affinity purified by Protein G

Forme physique

Phosphate-buffered saline, no preservative added.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins.
Schmidt TG and Skerra A
Nature Protocols, 2(6), 1528-1528 (2007)
Zhaofeng Yan et al.
Nature communications, 11(1), 241-241 (2020-01-15)
A major component of bacterial biofilms is curli amyloid fibrils secreted by the curli biogenesis system. Understanding the curli biogenesis mechanism is critical for developing therapeutic agents for biofilm-related infections. Here we report a systematic study of the curli biogenesis
The Strep-tag system for one-step affinity purification of proteins from mammalian cell culture.
Schmidt T and Skerra A
Methods in Molecular Biology, 1286, 83-95 (2015)
Timothy A Bates et al.
Cell reports, 34(7), 108737-108737 (2021-02-06)
In the ongoing coronavirus disease 2019 (COVID-19) pandemic, there remain unanswered questions regarding the nature and significance of the humoral immune response toward other coronavirus infections. Here, we investigate the cross-reactivity of antibodies raised against the first severe acute respiratory
Yongqiang Lai et al.
Scientific reports, 9(1), 14257-14257 (2019-10-05)
Replication protein A (RPA) binds to and stabilizes single-stranded DNA and is essential for the genome stability. We reported that an E3 ubiquitin ligase, HERC2, suppresses G-quadruplex (G4) DNA by regulating RPA-helicase complexes. However, the precise mechanism of HERC2 on

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