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Key Documents

SAB2702197

Sigma-Aldrich

Monoclonal Anti-GFP antibody produced in mouse

clone GT859, affinity isolated antibody

Synonyme(s) :

Mouse Monoclonal Anti-Green Fluorescent Protein

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

GT859, monoclonal

Forme

buffered aqueous solution

Espèces réactives

mouse

Concentration

1mg/mL

Technique(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1000-10000

Isotype

IgG2b

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Green fluorescent protein (GFP) is a fluorescent protein. It is mainly isolated from the luminous organ of the jellyfish Aequorea victoria.

Immunogène

Full length GFP recombinant protein

Application

Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, ICC/IF: 1:100-1:2000, IHC-Fr: Assay-dependent dilution, IHC-P: Assay-dependent dilution, IP: 1:100-1:500, WB: 1:1000-1:10000, IHC-Wm: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Monoclonal Anti-GFP antibody has been used in gait analysis.

Actions biochimiques/physiologiques

Green fluorescent protein (GFP) is used as a marker for gene expression. It is also used as a tag to study about protein localization in several organisms. GFP can form the intrinsic chromophore without the help of cofactors or enzymatic components.

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Autres remarques

Purification: Affinity purified by Protein G

Forme physique

Phosphate-buffered saline, no preservative added.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Manganese-induced trafficking and turnover of GPP130 is mediated by sortilin
Venkat S and Linstedt A D
Molecular Biology of the Cell, 28(19), 2569-2578 (2017)
Fluorescence-based biosensors: from concepts to applications
Progress in Molecular Biology and Translational Science, 113(1) (2012)
FACS-optimized mutants of the green fluorescent protein (GFP)
Cormack B P, et al.
Gene, 173(1), 33-38 (1996)
Zac1/GPR39 phosphorylating CaMK-II contributes to the distinct roles of Pax3 and Pax7 in myogenic progression
Yang Q, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(2), 407-419 (2018)
Yang Yang et al.
Nature neuroscience, 19(10), 1348-1355 (2016-09-07)
Neural circuits underlying auditory fear conditioning have been extensively studied. Here we identified a previously unexplored pathway from the lateral amygdala (LA) to the auditory cortex (ACx) and found that selective silencing of this pathway using chemo- and optogenetic approaches
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