Accéder au contenu
Merck
Toutes les photos(1)

Principaux documents

Voir toute la documentation

S2890

Sigma-Aldrich

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii

lyophilized powder, ≥700 DEA units/mg protein

Synonyme(s) :

Streptavidin-AP, Streptavidin−AP from Streptomyces avidinii

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro MDL:
MFCD00132384
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Conjugué

alkaline phosphatase conjugate

Niveau de qualité

200

Forme

lyophilized powder

Activité spécifique

≥700 DEA units/mg protein

Composition

Protein, ≥40% biuret

Température de stockage

−20°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Streptavidin, a 58 kDa protein, is a homotetramer purified from Streptomyces avidinii. It has a high affinity for biotin. Streptavidin has been conjugated with either peroxidase or alkaline phosphatase (ALP). These enzymes are used as protein conjugates because of their comparative stability and a wide range of suitable substrates for different applications. ALP conjugates to streptavidin in immunoassays.

Application

Streptavidin-alkaline phosphatase from Streptomyces avidinii has been used in enzyme-linked immune absorbent spot (ELISpot) to detect interferon (IFN-γ) producing cells.
The optimal working dilution should be determined empirically using a range of dilutions from a 1 mg/ml stock in buffer.

Définition de l'unité

One alkaline phosphatase unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

Forme physique

Lyophilized powder containing trehalose with EPPS, phosphate and traces of magnesium chloride and zinc chloride.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Matured tolerogenic dendritic cells effectively inhibit autoantigen specific CD4+ T cells in a murine arthritis model
Jansen MAA, et al.
Frontiers in Immunology, 10, 2068-2068 (2019)
Sialoside specificity of the siglec family assessed using novel multivalent probes: identification of potent inhibitors of myelin-associated glycoprotein.
Blixt O et al
The Journal of Biological Chemistry, 278(33), 31007-31019 (2003)
Production and characterization of virus-like particles and the P domain protein of GII.4 norovirus.
Koho T et al
Journal of Virological Methods, 179(1), 1-7 (2012)
Development and validation of sandwich quantitative ELISA prototypebased on the bovine IFNg for the detection of cellular immunity
Genc O, et al.
Turkish Journal of Veterinary and Animal Sciences, 39(6), 724-729 (2015)
Ola Blixt et al.
The Journal of biological chemistry, 278(33), 31007-31019 (2003-05-30)
Ten of the 11 known human siglecs or their murine orthologs have been evaluated for their specificity for over 25 synthetic sialosides representing most of the major sequences terminating carbohydrate groups of glycoproteins and glycolipids. Analysis has been performed using
Afficher tous les articles scientifiques apparentés

Protocoles

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique