R8903

Monoclonal Anti-hnRNP-K/J antibody produced in mouse

clone 3C2, purified immunoglobulin, buffered aqueous solution

• Synonyme(s) : Anti-Heterogeneous Nuclear Ribonucleoprotein K/J
• Numéro MDL: MFCD06798704
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 3C2, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~60 kDa
• Espèces réactives: hamster, Xenopus, bovine, human
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.1-0.2 μg/mL using total cell extract of MBDK cells
• Isotype: IgG2b
• Numéro d'accès UniProt: P61978
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... HNRNPK(3190)

Description

Description générale

Monoclonal Anti-hnRNP-K/J (mouse IgG2b isotype) is derived from the 3C2 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with hnRNP′s purified by oligo (dC) affinity chromatography.

Immunogène

hnRNPs purified by oligo (dC) affinity chromatography.

Application

The antibody may be used in various immunochemical techniques including enzyme linked immunosorbent assay (ELISA), immunoblotting, chromatin immunoprecipitation (ChIP), immunocytochemistry, antibody-based supershift assay and electrophoretic mobility shift assay (EMSA).

Actions biochimiques/physiologiques

Heterogeneous nuclear ribonucleoproteins (hnRNPs) act in several biological activities such as transcription, pre- mRNA processing, cytoplasmic mRNA translation and turnover. hnRNP-K is found both in the nucleus, cytoplasm, and mitochondria, therefore, it was found to be involved in many biological events in the cell like: transcription, chromatin remodeling, splicing and translation. hnRNP-K is regulated by different signaling cascades and can bind kinases. As a consequence, it can mediate signals from the cytoplasm to the nucleus.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable