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PIR01

Sigma-Aldrich

Inhibitor Resistant Genotyping PCR ReadyMix, Low ROX

with Low ROX for ABI and Stratagene instruments

Synonyme(s) :

Inhibitor Resistant Genotyping PCR ReadyMix

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About This Item

Code UNSPSC :
41106300
Nomenclature NACRES :
NA.55

Forme

liquid

Utilisation

sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions

Caractéristiques

Difficult Templates/Specialty Enzymes PCR
dNTPs included
hotstart

Conditions de stockage

protect from light

Technique(s)

qPCR: suitable

Couleur

light blue

Entrée

crude DNA

Méthode de détection

probe-based

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Description générale

Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use master mix for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.

A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hotstart allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Application

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:
  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Applied Biosystems ViiA 7
  • Stratagene Mx3000P®
  • Stratagene Mx3005P®
  • Stratagene Mx4000

Caractéristiques et avantages

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required
  • Compatible with both fast and conventional PCR cycling protocols

Composants

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, insert blue qPCR dye, and stabilizers.

250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Informations légales

Mx3000P is a registered trademark of Agilent Technologies, Inc.
Mx3005P is a registered trademark of Agilent Technologies, Inc.
Mx4000 is a trademark of Agilent Technologies, Inc.
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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