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PEROX1

Sigma-Aldrich

Peroxisome Isolation Kit

isolate peroxisomes from tissues and cells

Synonyme(s) :

Isolation Kit for Peroxisomes, Kit for Peroxisomes, Peroxisome Kit

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Niveau de qualité

200

Technique(s)

centrifugation: suitable
fractionation: suitable

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Catégories apparentées

Description générale

Isolated peroxisomes are used for studying lipid β-oxidation, amino acid metabolism and biosynthesis of ether-linked glycerolipids and bile acids.

Application

The Perixosome Isolation Kit provides all the necessary reagents and a detailed protocol for the isolation of highly purified peroxisomes from animal tissues and cells, by differential density gradient centrifugation using iodixanol [OptiPrep™]. This kit has been used for preparation of peroxisomes from rat liver, rat kidney and rabbit liver as well as HEK293 and HepG2 cells.

Caractéristiques et avantages

  • Specially formulated extraction reagents for research scale applications - save time and minimize waste
  • Produces functional intact organelles - resulting peroxisomes are suitable for functional studies, metabolic assays, protein profiling, and disease state analysis
  • Compatible with products for structure confirmation - easily confirm intactness with companion test kit, Cytochrome C Reductase Assay Kit (Cat. No. CY0100)

Autres remarques

Upon receiving the kit, the Protease Inhibitor Cocktail (Product Code P 8340) should be stored at –20 °C and the OptiPrep Density Gradient Medium (Product Code O3028) should be stored at room temperature.

Informations légales

OptiPrep is a trademark of Serumwerk Bernburg AG

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionFDS

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290,H315,H319

Classification des risques

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
048M4160V
11180104
017M4126V
11160527
045M4050V

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M Y Kang et al.
Cell death and differentiation, 20(1), 117-129 (2012-08-25)
The tumor suppressor p53 is an important regulator of intracellular reactive oxygen species (ROS) levels, although downstream mediators of p53 remain to be elucidated. Here, we show that p53 and its downstream targets, p53-inducible ribonucleotide reductase (p53R2) and p53-inducible gene
M Une et al.
Journal of lipid research, 37(12), 2550-2556 (1996-12-01)
The oxidation of the side chains of two potential bile acid intermediates, 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid (THCA) and 3 alpha,7 alpha-dihydroxy-5 beta-cholestanoic acid (DHCA), were investigated in rat liver mitochondria and peroxisomes. Both THCA and DHCA were efficiently
G P Mannaerts et al.
Biochimie, 75(3-4), 147-158 (1993-01-01)
This article summarizes our current knowledge of the metabolic pathways present in mammalian peroxisomes. Emphasis is placed on those aspects that are not covered by other articles in this issue: peroxisomal enzyme content and topology; the peroxisomal beta-oxidation system; substrates
K Burdett et al.
The Journal of biological chemistry, 266(19), 12201-12206 (1991-07-05)
Upon differential centrifugation of guinea pig intestine mucosal cells homogenate, fatty acyl-CoA:NADPH oxidoreductase (long chain alcohol forming) was found to be enriched in the light mitochondrial (L) fraction (sedimenting between 66,000 x g min and 500,000 x g min) which
P B Lazarow et al.
Proceedings of the National Academy of Sciences of the United States of America, 73(6), 2043-2046 (1976-06-01)
Purified rat liver peroxisomes contain a cyanide-insensitive fatty acyl-CoA oxidizing system that uses O2 and NAD as electron acceptors. The system was detected by the ability of added palmitoyl-CoA to elicit O2 consumption, H2O2 production, and O2-dependent NAD reduction. The
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