PCRISPR006

Human CRISPR Poison Exon Knockout Library

Propriétés

• Conditionnement: pkg of 5 vials (5x200µL aliquots )
• Concentration: ≥5x108 VP/ml (via p24 Assay)
• Application(s): CRISPR
• Conditions d'expédition: dry ice
• Température de stockage: −70°C

Description

Description générale

The human, paired guide Poison (pgPoison) library targets 3` splice sites with paired guide RNAs for alternative exon removal (pgRNAs), induce skipping of "poison" cassette exons and corresponding upstream constitutive exons in ultraconserved regions. This compact library (targets 963 exons) allows direct comparison between loss of a constitutive coding exon (knockout) with loss of a poison exon.

Custom pools for follow-up screening or 10x Genomics Compatible CRISPR pools are also available by contacting your local sales representative.

Application

• Functional Genomics/Target Validation
• Dual-gRNA
• Poison exon
• Internal controls included

Caractéristiques et avantages

• Focus on your research, and we will generate your lentivirus screening library
• Use CRISPR nucleases to knockout protein-coding genes to assess their function.
• Built-in enrichment and depletion controls allow researchers to gauge the success of their pooled screening experiments confidently.
• Dual-Guide Vector System (pools are gRNA-only, Cas9 sold separately) See products: LVCAS9BST or LVCAS9NEO.
• Ease of optimization: Utilizes BFP and Puromycin as selection markers under EF1alpha promoter.

Principe

In traditional CRISPR KO screens, Cas9 introduces double-strand breaks at locations specified by a gRNA. In this library, a CRISPR-Cas9-based method is used by expressing two sgRNAs to manipulate isoforms independent of gene inactivation. This approach enables rapid suppression of exon recognition in polyclonal settings to identify functional roles for individual exons.

Informations légales

CRISPR USE LICENSE AGREEMENT

LENTIVIRAL, WPRE AND EVROGEN FLUOROPHORE LICENSES

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable