Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

P7059

Sigma-Aldrich

Solution tampon phosphate

10X, liquid, pH 7.2-7.6 (1x solution), for molecular biology

Synonyme(s) :

PBS

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro MDL:
MFCD00131855
Code UNSPSC :
12161700
eCl@ss :
32129211
Nomenclature NACRES :
NA.25

product name

Solution tampon phosphate, 10× PBS for Western blots and IP

Qualité

for molecular biology

Niveau de qualité

200

Stérilité

0.2 μm filtered

Forme

liquid

Technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

pH

7.2-7.6(1x solution)

Température de stockage

room temp

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

Application

Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.

Composants

10x PBS contains:
  • 30mM sodium phosphate
  • 1.5 M sodium chloride
  • 10.5 mM potassium phosphate

Notes préparatoires

Dilute 10x PBS to a 1x working solution using molecular biology grade water.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Imran Tarique et al.
Animals : an open access journal from MDPI, 9(11) (2019-11-07)
The ductuli efferentes (DE) form a transit passage for the passage of spermatozoa from the rete testis to the epididymis. After spermiation, various epithelial secretory proteins are transferred via extracellular vesicles (EVs) to the spermatozoa for their maturation and long-term
Sahar M Greish et al.
International journal of physiology, pathophysiology and pharmacology, 12(5), 134-146 (2020-11-24)
Hypertension can be induced by inhibiting nitric oxide synthesis with L-NAME, which also has a role in oxidative stress. Curcumin has strong antioxidant property. Our aim was to examine the possible preventive role of curcumin on renal dysfunction secondary to
Jennifer L S Sporty et al.
Analytical chemistry, 82(15), 6593-6600 (2010-07-14)
A novel method for extracting butyrylcholinesterase (BuChE) from serum as a means of identifying and measuring nerve agent adducts to human BuChE is presented here. Antibutyrylcholinesterase monoclonal antibodies were conjugated to protein-G ferromagnetic particles and mixed with 500 microL serum
Johan E Karlsson et al.
Drug metabolism and disposition: the biological fate of chemicals, 38(4), 705-714 (2010-01-15)
Membrane-bound transporter proteins play an important role in the efflux of drugs from cells and can significantly influence the pharmacokinetics of drug molecules. This study describes the production of large amounts of high-activity transporter membrane vesicles from human embryonic kidney
Caroline R Archer et al.
Scientific reports, 8(1), 10160-10160 (2018-07-07)
Pharmaceutical agents despite their efficacy to treat disease can cause additional unwanted cardiovascular side effects. Cardiotoxicity is characterized by changes in either the function and/or structure of the myocardium. Over recent years, functional cardiotoxicity has received much attention, however morphological
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique