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P5412

Sigma-Aldrich

o-Phenylenediamine

peroxidase substrate, chromogenic, tablet

Synonyme(s) :

1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD

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About This Item

Formule empirique (notation de Hill):
C6H8N2
Numéro CAS:
95-54-5
Poids moléculaire :
108.14
Numéro Beilstein :
606074
Numéro CE :
202-430-6
Numéro MDL:
MFCD00007721
Code UNSPSC :
12352204
ID de substance PubChem :
24898638
Nomenclature NACRES :
NA.83

product name

o-Phenylenediamine, tablet, 20 mg substrate per tablet

Densité de vapeur

3.7 (vs air)

Pression de vapeur

0.01 mmHg ( 25 °C)

Forme

tablet

Point d'ébullition

256-258 °C

Pf

100-102 °C

Fluorescence

λex 337 nm; λem 417 nm (after derivatization)

Température de stockage

2-8°C

Chaîne SMILES 

Nc1ccccc1N

InChI

1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2

Clé InChI

GEYOCULIXLDCMW-UHFFFAOYSA-N

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Application

o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
o-Phenylenediamine has been used to detect transforming growth factor (TGF-β) and to measure anti-cTfRMAb-ScFv fusion protein using enzyme linked immunosorbent assay (ELISA).

Actions biochimiques/physiologiques

o-Phenylenediamine (o-PD) is a potent substrate for spectrophotometric horseradish peroxidase (HRP)-mediated enzyme-linked immunosorbent assay (ELISA), which gives 2,3-diaminophenazine (DAP) as a product of o-PD oxidation.

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Pictogrammes

Skull and crossbonesHealth hazardEnvironment
GHS06,GHS08,GHS09

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

276.8 °F - closed cup

Point d'éclair (°C)

136 °C - closed cup

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Application of o-phenylenediamine as a fluorogenic substrate in peroxidase-mediated enzyme-linked immunosorbent assay
Mekler VM and Bystryak SM
Analytica Chimica Acta, 264(2), 359-363 (1992)
Receptor-mediated abeta amyloid antibody targeting to Alzheimer?s disease mouse brain
Zhou QH, et al.
Molecular Pharmaceutics, 8(1), 280-285 (2010)
Interleukin-13 Regulates Secretion of the Tumor Growth Factor-beta Superfamily Cytokine Activin A in Allergic Airway Inflammation
Hardy CL, et al.
American Journal of Respiratory Cell and Molecular Biology, 42(6), 667-675 (2010)
Jonas R Knudsen et al.
International journal of molecular sciences, 21(7) (2020-04-02)
The small molecule kinase inhibitor SBI-0206965 was originally described as a specific inhibitor of ULK1/2. More recently, it was reported to effectively inhibit AMPK and several studies now report its use as an AMPK inhibitor. Currently, we investigated the specificity
Breno S Diniz et al.
The journals of gerontology. Series B, Psychological sciences and social sciences, 69(6), 845-851 (2014-08-26)
Late-life depression (LLD) is associated with reduced neurotrophic support and abnormalities in neurodegenerative cascades. The aim of the present study is to determine the concentrations of brain-derived neurotrophic factor (BDNF), amyloid-β42, total Tau, and phosphorylated Tau in the cerebrospinal fluid
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Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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