P0089

Anti-Prox1 antibody , Mouse monoclonal

clone 4G10, purified from hybridoma cell culture

• Synonyme(s) : Anti-Homeobox prospero-like protein Prox1, Anti-Prospero homeobox 1
• Numéro MDL: MFCD09039204
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: 4G10, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~84 kDa
• Espèces réactives: chicken, rat, lizard, human
• Concentration: ~1.5 mg/mL
• Technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HepG2 cells; western blot: suitable
• Isotype: IgG2b
• Numéro d'accès UniProt: Q92786
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PROX1(5629) ; rat ... Prox1(305066)

Description

Description générale

Monoclonal Anti-Prox1 (mouse IgG2b isotype) is derived from the hybridoma 4G10 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant fusion protein of human Prox1. Prospero related homeobox gene 1 (Prox1) belongs to transcription factor subfamily and is homologous to Drosophila homeobox protein, prospero.

Immunogène

recombinant fusion protein corresponding to amino acids 547-737 of human Prox1.

Application

Anti-Prox1 antibody, Mouse monoclonal has been used in various immunochemical techniques.

Actions biochimiques/physiologiques

Prospero related homeobox gene 1 (Prox1) is a key regulator for neuroblast fate determination and photoreceptor development. Prox1 regulates cell differentiation and organogenesis in lens, retina, lymphatic vessels, liver and pancreas. It was found to act as a tumor suppressor gene, because its deficiency or its mutated forms have been implicated in various human cancers. Knockdown of Prox1 by siRNA or by RNA mutations significantly accelerated the in vitro and in vivo growth of hepatocellular carcinoma (HCC) as well as esophageal cancer cells. Prox1 is hypermethylated and transcriptionally silenced in primary and metastatic breast cancer.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)