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Key Documents

OGS3157

Sigma-Aldrich

PSF-OXB20-NH2-PELB - PELB SECRETION PLASMID

plasmid vector for molecular cloning

Synonyme(s) :

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.85

Produit recombinant

expressed in E. coli

Forme

buffered aqueous solution

Poids mol.

size 3914 bp

Sélection de bactéries

kanamycin

Origine de la réplication

pUC (500 copies)

Clivage des peptides

no cleavage

Localisation du marqueur peptidique

N-terminal

Promoteur

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

Gène reporter

none

Signal de sécrétion

PelB

Conditions d'expédition

ambient

Température de stockage

−20°C

Description générale

This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.

About the Peptide Tag:This plasmid contains an Pectate Lyase B (PelB) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.

Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

Séquence

To view sequence information for this product, please visit the product page

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Produit(s) apparenté(s)

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PSF-CMV-PURO-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG MAMMALIAN PLASMID, plasmid vector for molecular cloning

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PSF-CMV-PURO-NH2-FLAG®-6HIS-EKT - N-TERMINAL FLAG® AND 6 HIS DUAL TAG MAMMALIAN PLASMID, plasmid vector for molecular cloning

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PSF-TEF1-NH2-FLAG®-6HIS-EKT - N-TERMINAL FLAG® AND 6 HIS DUAL TAG YEAST PLASMID, plasmid vector for molecular cloning

OGS1913

PSF-TEF1-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG YEAST PLASMID, plasmid vector for molecular cloning

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PSF-OXB20-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG BACTERIAL PLASMID, plasmid vector for molecular cloning

OGS2828

PSF-OXB20-NH2-FLAG®-6HIS-EKT - N-TERMINAL FLAG® AND 6 HIS DUAL TAG BACTERIAL PLASMID, plasmid vector for molecular cloning

OGS394

PSF-CMV-PGK-PURO - DUAL PROMOTER PUROMYCIN PLASMID, plasmid vector for molecular cloning

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PSF-OXB20-FLUC - BACTERIAL LUCIFERASE PLASMID, plasmid vector for molecular cloning

OGS502

PSF-LACI - LACI PROMOTER BACTERIAL VECTOR, plasmid vector for molecular cloning

OGS505

PSF-CAG-KAN - CAG PROMOTER VECTOR, plasmid vector for molecular cloning

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PSF-OXB1 - WEAK STRENGTH BACTERIAL PROMOTER PLASMID, plasmid vector for molecular cloning

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PSF-CMV-BLAST - CMV PROMOTER BLASTICIDIN RESISTANT PLASMID, plasmid vector for molecular cloning

PP2356

Mammalian Promoter Vector Set, plasmid vectors for molecular cloning

PP2381

Yeast Signal Peptide Vector Set, plasmid vectors for molecular cloning

PP2393

Mammalian FLAG® Tag Vector Set, plasmid vectors for molecular cloning

PP2394

Bacterial FLAG® Tag Vector Set, plasmid vectors for molecular cloning

B11001

EnPresso® B, Growth system for expressing protein in bacteria

CMC0001

SIG10 Chemically Competent Cells, for protein expression and DNA plasmid production

CMC0007

SIG10 5a Chemically Competent Cells, for DNA plasmid production

CMC0014

BL21(DE3) Chemically Competent Cells, for protein expression

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

Code de la classe de stockage

12 - Non Combustible Liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
Jin-Gyoung Jung et al.
PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.
Diana Romero et al.
Carcinogenesis, 37(1), 18-29 (2015-10-28)
Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
Alexander C Cerny et al.
PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual

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