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NDEGLY

Sigma-Aldrich

Native Protein Deglycosylation Kit

Synonyme(s) :

Protein Deglycosylation Kit

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Conjugué

(N-linked)

Niveau de qualité

200

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

All complex oligosaccharides can be reduced to the trimannosylchitobiose core by treatment of the glycoproteins with neuraminidase, β-galactosidase, and N-acetylglucosaminidase. Fucosidases may be required in some situations. The remaining trimannosylchitobiose core structures can be removed with endoglycosidase F3. Biantennary and triantennary structures can be immediately removed by endoglycosidases F2 and F3, respectively. Oligomannose and hybrid structures can be removed by Endoglycosidase F1.
For more information on each type of endoglycosidase, please refer to the Bulletin.
The Native Protein Deglycosylation is designed for the deglycoslylation of N-linked oligosaccharides from PNGase F-resistant native proteins. Endoglycosidases F1, F2, and F3 are less sensitive to protein conformation than PNGase F and are more suitable for removal of all classes of N-linked oligosaccharides without protein denaturation.

Application

Native Protein Deglycosylation Kit has been for N-deglycosylation of various enzymes such as laccase, tomato nuclease TBN1 and endo-β-1,3-glucanase.

Stockage et stabilité

The NDEGLY Kit ships on wet ice and storage at 2–8 °C is recommended. This kit may be used for at least 1 year when stored as indicated. Several days exposure to ambient temperatures will not reduce the activity of the enzymes in this kit.

Composants de kit seuls

Réf. du produit
Description
  • Endoglycosidase F1 .3 U
  • Endoglycosidase F2 .1 U
  • Endoglycosidase F3 .1 U
  • Endoglycosidase F1 reaction buffer 200 μL
  • Endoglycosidase F2 & 3 reaction buffer 200 μL

Code de la classe de stockage

12 - Non Combustible Liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

N-glycosylation of tomato nuclease TBN1 produced in N. benthamiana and its effect on the enzyme activity
Podzimek T, et al.
Plant Science (2018)
Junyi Ma et al.
Molecular pharmacology, 69(4), 1137-1145 (2006-01-10)
Opioid analgesics remain the choice for the treatment of moderate to severe pain. Recent research has established that the mu-opioid receptor is predominantly responsible for mediating many opioid actions, including analgesia and opioid tolerance. However, the function of delta-opioid receptors
Adela Rodríguez-Romero et al.
Acta crystallographica. Section D, Biological crystallography, 70(Pt 2), 329-341 (2014-02-18)
Endogenous glycosylated Hev b 2 (endo-β-1,3-glucanase) from Hevea brasiliensis is an important latex allergen that is recognized by IgE antibodies from patients who suffer from latex allergy. The carbohydrate moieties of Hev b 2 constitute a potentially important IgE-binding epitope
Chaouki Benabdessalem et al.
Biochemical and biophysical research communications, 516(3), 845-850 (2019-07-03)
We previously reported that immunoreactivity of recombinant CFP32 (Rv0577), a virulence factor of Mycobacterium tuberculosis, was higher when produced in transformed Pichia pastoris as compared to transformed E. coli. In this study, we show that this difference is partly due to
M P Kolomytseva et al.
International journal of biological macromolecules, 137, 1199-1210 (2019-07-12)
Three laccase isoforms with different physicochemical properties could be purified from culture liquid of basidiomycete Lentinus strigosus 1566 obtained during submerged cultivation. The purified laccases possessed individual selectivity in relation to different phenolic compounds. Laccases I, II, and III (59
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Articles

O-Linked Glycan Strategies

There is no enzyme comparable to PNGase F for removing intact O-linked sugars. Monosaccharides must be sequentially hydrolyzed by a series of exoglycosidases until only the Gal-b(1-3)-GalNAc core remains. O-Glycosidase can then remove the core structure intact with no modification of the serine or threonine residue.

Endoglycosidases

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