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NA0310

Sigma-Aldrich

GenElute™ HP Plasmid Maxiprep Kit

greener alternative

sufficient for 25 purifications

Synonyme(s) :

GenElute™ HP Plasmid Maxiprep Kit, HP maxiprep kit, fast maxiprep kit, fast plasmid isolation, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, ultrafast plasmid isolation

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About This Item

Code UNSPSC :
41105501
Nomenclature NACRES :
NA.52

Utilisation

sufficient for 25 purifications

Niveau de qualité

200

Caractéristiques du produit alternatif plus écologique

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Technique(s)

DNA purification: suitable

Autre catégorie plus écologique

Aligned,

Température de stockage

15-25°C

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Catégories apparentées

Description générale

The GenElute HP Plasmid Maxiprep Kit offers a simple, rapid, and cost effective method for isolating plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format. Up to 1.2 mg of plasmid DNA can be isolated from a 150 mL overnight culture grown in Luria Broth (LB) medium. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.

The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

GenElute® HP Plasmid Maxiprep Kit has been used in the isolation of plasmids.

Caractéristiques et avantages

  • From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
  • Up to 1.2 mg of high-copy plasmid DNA
  • Flexibility of a vacuum or spin format
  • Contains fewer plastic components than other high speed kits, reducing the amount of waste
  • No phenol/chloroform extraction or alcohol precipitation required

Autres remarques

For additional information, please see www.sigma-aldrich.com/genelutehp.

Informations légales

Gabitril is a registered trademark of Abbott Laboratories Corp.
GenElute is a trademark of Sigma-Aldrich Co. LLC

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

Organes cibles

Central nervous system

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

77.0 °F - closed cup

Point d'éclair (°C)

25 °C - closed cup

Certificats d'analyse (COA)

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Jaeho Yoon et al.
Biomedicines, 9(9) (2021-09-29)
Renal hypouricemia is a rare genetic disorder. Hypouricemia can present as renal stones or exercise-induced acute renal failure, but most cases are asymptomatic. Our previous study showed that two recessive variants of SLC22A12 (p.Trp258*, pArg90His) were identified in 90% of
Musaddeque Ahmed et al.
Nature communications, 12(1), 1781-1781 (2021-03-21)
Prostate cancer (PCa) risk-associated SNPs are enriched in noncoding cis-regulatory elements (rCREs), yet their modi operandi and clinical impact remain elusive. Here, we perform CRISPRi screens of 260 rCREs in PCa cell lines. We find that rCREs harboring high risk
Discovery of protein interactions using parallel analysis of translated ORFs (PLATO).
Larman HB
Nature Protocols, 9(1), 90-103 (2014)
Fraser Soares et al.
Blood, 137(16), 2171-2181 (2020-12-04)
Acute myeloid leukemia (AML) remains a devastating disease in need of new therapies to improve patient survival. Targeted adoptive T-cell therapies have achieved impressive clinical outcomes in some B-cell leukemias and lymphomas but not in AML. Double-negative T cells (DNTs)
Ilaria Mogno et al.
Genome research, 23(11), 1908-1915 (2013-08-08)
Gene promoters typically contain multiple transcription factor binding sites (TFBSs), which may vary in affinity for their cognate transcription factors (TFs). One major challenge in studying cis-regulation is to understand how TFBS variants affect gene expression. We studied the in
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Protocoles

GenElute™ HP Plasmid Maxiprep procedure

Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.

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