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N5389

Sigma-Aldrich

Monoclonal Anti-Neurofilament 200 antibody produced in mouse

enhanced validation

clone NE14, ascites fluid

Synonyme(s) :

NF200 Antibody - Monoclonal Anti-Neurofilament 200 antibody produced in mouse, Neurofilament Antibody, Nf200 Antibody, Monoclonal Anti-Neurofilament heavy chain

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About This Item

Numéro MDL:
MFCD02263444
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

NE14, monoclonal

Poids mol.

antigen apparent mol wt 200 kDa

Contient

15 mM sodium azide as preservative

Espèces réactives

pig, mouse, chicken, bovine, guinea pig, rabbit, rat, human

Validation améliorée

independent ( Antibodies)
Learn more about Antibody Enhanced Validation

Technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
immunohistochemistry (frozen sections): suitable
microarray: suitable
western blot: suitable

Isotype

IgG1

Numéro d'accès UniProt

P12036

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... NEFH(4744)

Description générale

Monoclonal Anti-Neurofilament 200 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The neurofilaments are one of the five major groups of intermediate filaments (IFs) and are found predominantly in cells or tissues of neuronal origin. They are composed of three major proteins of apparent molecular weights 68 kD, 160 kD, and 200 kD and are named as NEFL (light), NEFM (medium) and NEFH (heavy) respectively. Neurofilament proteins are synthesized in the neuronal perikarya, assembled to form filaments and then slowly transported within the axons towards the synaptic terminals.

Immunogène

pig spinal cord.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-Neurofilament 200 antibody produced in mouse has been used in
  • immunohistochemistry
  • immunolabeling
  • immunofluorescence

Actions biochimiques/physiologiques

Neurofilament heavy chain (NEFH) gene was recently identified to cause autosomal dominant axonal Charcot-Marie-Tooth disease (CMT2cc). This gene plays a role in the pathogenesis of sporadic amyotrophic lateral sclerosis (ALS). The phosphorylation of neurofilament polypeptides has been suggested to modulate their function by influencing the interaction between neurofilament and cytoplasmic organelles.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Cell cycle kinetics and immunohistochemical characterization of dissociated fetal neocortical cultures: evidence that differentiated neurons have mitotic capacity
Jacobs JS and Miller MW
Dev. Brain Res., 122(1), 67-80 (2000)
Neurofilament proteins are synthesized in nerve endings from squid brain
Crispino M, et al.
Journal of Neurochemistry, 61(3), 1144-1146 (1993)
Pamela Imperadore et al.
Scientific reports, 7, 46564-46564 (2017-04-21)
Regeneration is a process that restores structure and function of tissues damaged by injury or disease. In mammals complete regeneration is often unsuccessful, while most of the low phyla animals can re-grow many parts of their body after amputation. Cephalopod
C Lois et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(5), 2074-2077 (1993-03-01)
Subventricular zone (SVZ) cells proliferate spontaneously in vivo in the telencephalon of adult mammals. Several studies suggest that SVZ cells do not differentiate after mitosis into neurons or glia but die. In the present work, we show that SVZ cells
Jon O Cleary et al.
NeuroImage, 56(3), 974-983 (2011-02-12)
Extensive worldwide efforts are underway to produce knockout mice for each of the ~25,000 mouse genes, which may give new insights into the underlying pathophysiology of neurological disease. Microscopic magnetic resonance imaging (μMRI) is a key method for non-invasive morphological
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