N4412

Anti-Neurabin I antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-Neural-tissue Specific F-actin Binding Protein I, Anti-Protein Phosphatase 1 Regulatory Subunit 9A
• Numéro MDL: MFCD02263807
• Code UNSPSC : 12352203

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 180 kDa
• Espèces réactives: mouse, rat
• Technique(s): microarray: suitable; western blot: 0.5-1 μg/mL using a rat brain extract (S1 fraction); western blot: 1-2 μg/mL using mouse brain (fraction S1).
• Numéro d'accès UniProt: O35867
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Ppp1r9a(243725) ; rat ... Ppp1r9a(84685)

Description

Description générale

Neurabin I is expressed in the central nervous system of rats.

Neurabin I or neural tissue-specific F-actin-binding protein is highly enriched in dendritic spines. It comprises the F-actin binding domain at the N-terminus, a single PDZ interacting-domain and a C-terminal coiled-coil domain. Neurabin I is localized in the synapse of mature neurons and the lamellipodia of the growth cone during neuronal development.

Spécificité

Anti-Neurabin I specifically recognizes neurabin I (180 kDa).

Immunogène

synthetic peptide located near the N-terminus of rat neurabin I (amino acids 341-361). This sequence is highly conserved (80%) in mouse neurabin I and not found in neurabin II/spinophilin.

Application

Anti-Neurabin I antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Actions biochimiques/physiologiques

Neurabin I has a role in the assembly of F-actin. It has been shown to be associated with stimulation of hepatocarcinogenesis in rats.

Neurabin I is essential for neurite formation and binds to several proteins including F-actin, protein phosphatase 1 (PP1), p70 S6 kinase (p70S6K) and TGN38. Neurabin I/PP1 complex has been suggested to play a role in actin cytoskeleton dynamics and also in the control of cell morphology in mammalian neurons. The interaction of neurabin I with the trans-Golgi network membrane protein (TGN38) enables a link between TGN38-containing membranes and the actin cytoskeleton. Neurabin′s presence in fetal tissue may mediate early development, through regulation of actin cytoskeleton dynamics in the differentiation of skeletal muscle cells.

Description de la cible

Neurabin I (Neural-tissue specific F-actin bindingprotein I, PP1BP175, p180, also termed Proteinphosphatase 1 regulatory subunit 9A, PPP1R9A, 180kDa), belongs to a family of F-actin-binding proteins, including neurabin II/spinophilin, which are high

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable