MAK538

Ammonia Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.84

Propriétés

• Niveau de qualité: 100
• Méthode de détection: colorimetric; fluorometric
• Maladie(s) pertinente(s): gastrointestinal diseases
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Ammonia (NH3) or its ion form ammonium (NH4 +) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase).

Caractéristiques et avantages

• Linear detection range: 24 to 1000 µM

Adéquation

The kit is suitable for ammonia determination in biological samples (e.g. serum, plasma, urine, saliva, cell culture, etc).

Principe

The Ammonia Assay Kit is designed to directly measure NH₃ and NH4 +. In this assay, NADH is converted to NAD⁺ in the presence of NH₃, ketoglutarate and glutamate dehydrogenase. The decrease in optical density at 340 nm or fluorescence intensity at λEx = 360 nm/λEm = 450 nm is directly proportionate to the NH3 concentration in the sample.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3