MAK166

Fluorimetric Hydrogen Peroxide Assay Kit

sufficient for 500 fluorometric tests (infrared fluorescence)

• Numéro CE : 231-765-0
• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.84

Propriétés

• Utilisation: sufficient for 500 fluorometric tests (infrared fluorescence)
• Méthode de détection: fluorometric
• Maladie(s) pertinente(s): orthopedic diseases; cardiovascular diseases; aging/geriatric diseases; neurological disorders; pulmonary disorders
• Température de stockage: −20°C

Description

Description générale

Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

Application

Fluorimetric Hydrogen Peroxide Assay Kit has been sued to determine the oxidation pressure (H₂O₂).

Caractéristiques et avantages

This assay is compatible with high-throughput handling systems.

Adéquation

The Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.

Principe

Fluorimetric Hydrogen Peroxide Assay Kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates an infrared fluorescent product (λ_ex = 640/λ_em = 680 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader. The generation of the fluorescence product is pH independent between pH 4 to pH 10 and is superior to other fluorescence kits for measuring hydrogen peroxide in low pH solutions which often display decreased probe sensitivity. The infrared fluorescent probe is less sensitive to assay background due to autofluorescence from biological samples. This assay is compatible with high-throughput handling systems.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317 - H334
• Conseils de prudence: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classification des risques: Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable