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MAK117

Sigma-Aldrich

Glycerol Assay Kit

sufficient for 200 colorimetric or fluorometric tests

Synonyme(s) :

Glycerin Assay Kit, Glycerine Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 200 colorimetric or fluorometric tests

Application(s)

cosmetics
environmental
food and beverages

Méthode de détection

colorimetric
fluorometric

Maladie(s) pertinente(s)

cancer

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Glycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides. Glycerol is a sweet-tasting, hygroscopic liquid. Glycerol is widely used in food, beverage, and pharmaceutical formulations and is the main waste by-product of biodiesel production via transesterification.

Application

Glycerol assay kit has been used to quantify glycerol release from mouse 3T3-L1 cell culture supernatant samples and to measure plasma glycerol levels.

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

This kit is suitable for glycerol detection in foods, beverages, pharmaceutical formulations, and biological samples.

Principe

In this kit, glycerol concentration is determined by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the glycerol present. The linear range of detection for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids


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Consulter la Bibliothèque de documents

Martina Rudnicki et al.
Frontiers in physiology, 9, 1452-1452 (2018-11-09)
Background: Impaired capillary growth (angiogenesis) in skeletal muscle and adipose tissue contributes to the development of metabolic disorders in obese males. This association remains unexplored in females, despite mounting evidence that endothelial cells have sex-specific transcriptional profiles. Therefore, herein we
Meichun Zhang et al.
PLoS neglected tropical diseases, 13(2), e0007139-e0007139 (2019-02-05)
As an important vector of dengue and Zika, Aedes albopictus has been the fastest spreading invasive mosquitoes in the world over the last 3-4 decades. Cold tolerance is important for survival and expansion of insects. Ae. albopictus adults are generally
Nicolae S.
Health & Drugs: Disease, Prescription & Medication (2014)
Paula Dunayevich et al.
Scientific reports, 8(1), 15168-15168 (2018-10-13)
Cells make decisions based on a combination of external and internal signals. In yeast, the high osmolarity response (HOG) is a mitogen-activated protein kinase (MAPK) pathway that responds to a variety of stimuli, and it is central to the general
Sylvia J Gasparini et al.
Diabetologia, 62(8), 1463-1477 (2019-05-18)
Chronic glucocorticoid therapy causes insulin resistance, dyslipidaemia, abnormal fat accumulation, loss of muscle mass and osteoporosis. Here we describe a hitherto unknown sexual dimorphism in the metabolic response to chronic glucocorticoid exposure in mice. This led us to investigate whether

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