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MAK116

Sigma-Aldrich

Creatine Kinase Activity Assay Kit

sufficient for 100 tests (Ultraviolet)

Synonyme(s) :

CK Activity Assay Kit, Creatine Phosphokinase Activity Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 tests (Ultraviolet)

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

cardiovascular diseases; genitourinary disorders; aging/geriatric diseases; orthopedic diseases

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

human ... CKB(1152) , CKM(1158)
mouse ... CKB(12709) , CKM(12715)
rat ... CKB(24264) , CKM(24265)

Catégories apparentées

Description générale

Creatine Kinase (CK), also known as phosphocreatine kinase, is an enzyme that catalyzes the transfer of one phosphate group from ATP to creatine generating phosphocreatine, an important energy reservoir in muscle and brain tissue. CK is a dimeric protein made up of B (brain) and M (muscle) subunits. Three isoenzymes, CK-MM, CK-MB, and CK-BB, have been observed. CK levels are elevated in various pathological conditions including myocardial infarction, rhabdomyolysis, muscular dystrophy, and renal failure.

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

Suitable for the detection of Creatine Kinase activity in a variety of samples such as serum and plasma. This kit can be used to study the effects of various compounds on Creatine Kinase activity.

Principe

Creatine Kinase activity is determined by a coupled enzyme reaction resulting in the production of NADPH, measured at 340 nm, proportionate to the CK activity present in the sample. In this reaction, phosphocreatine and ADP are converted to creatine and ATP. The generated ATP is used by hexokinase to phosphorylate glucose resulting in glucose-6-phosphate, which is oxidized by NADP in the presence of glucose-6-phosphate dehydrogenase to produce NADPH and 6-phospho-D-gluconate. One unit of CK is the amount of enzyme that will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per minute at pH 6.0. This kit has a linear range of 30–1,800 units/L CK activity.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

06693

Timestrip Plus™ -20 °C

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Gary R Hunter et al.
Medicine and science in sports and exercise, 49(12), 2414-2421 (2017-07-25)
Resting energy expenditure (REE) increases after an intense exercise; however, little is known concerning mechanisms. The purpose of this study was to determine effects of a single bout of moderate-intensity continuous (MIC) aerobic exercise, or high-intensity interval (HII) exercise on
Crystal structure of rabbit muscle creatine kinase.
Rao JK, et al.
Febs Letters, 133-7 null
P2RX7 Purinoceptor: A Therapeutic Target for Ameliorating the Symptoms of Duchenne Muscular Dystrophy
Sinadinos A
PLoS Medicine null
Christopher W Bach et al.
European journal of applied physiology, 117(5), 913-919 (2017-03-18)
To investigate the blood glucose kinetics and physiological effects experienced by a type 1 diabetic (T1D) finisher of a 3-day, multi-stage ultra endurance triathlon consisting of a 10 km swim and 144.8 km bike (stage 1), a 275.4 km bike (stage 2), and
HANAC Col4a1 Mutation in Mice Leads to Skeletal Muscle Alterations due to a Primary Vascular Defect
Guiraud S, et al.
The American Journal of Pathology, 505-516 null
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