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MAK049

Sigma-Aldrich

Phosphatidylcholine Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonyme(s) :

Phosphatidylcholine Test Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric or fluorometric tests

Méthode de détection

colorimetric
fluorometric

Maladie(s) pertinente(s)

neurological disorders; gastrointestinal diseases; cancer

Température de stockage

−20°C

Description générale

Phosphatidylcholine (PC) accounts for more than 50% of the phospholipids composing mammalian plasma membranes and is particularly enriched in the extracellular leaflet. PC is synthesized in the liver and is required for lipoprotein assembly and secretion. PC is cleaved by phospholipase D, which hydrolyses the choline head, producing the signaling intermediate phosphatidic acid.

Application

Phosphatidylcholine Assay Kit has been used to determine the concentration of phosphatidylcholine in samples.

Adéquation

Suitable for the measurement of phosphatidylcholine in a variety of biological samples

Principe

In this assay, PC concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the PC present.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

Certificats d'analyse (COA)

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USP

USP

1535755

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Supelco

P3869

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Sigma-Aldrich

P3556

L-α-phosphatidylcholine

vibrant-m

Y0001905

Phosphatidylcholine from egg yolk

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Alena P, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1862(12), 1469-1480 (2017)
Riboflavin attenuates myocardial injury via LSD1-mediated crosstalk between phospholipid metabolism and histone methylation in mice with experimental myocardial infarction.
Peng W, et al.
Journal of Molecular and Cellular Cardiology, 115, 115-129 (2018)
Copper-64 labeled liposomes for imaging bone marrow.
Sang-gyu L, et al.
Nuclear Medicine and Biology, 43(12), 781-787 (2016)
Sang-Gyu Lee et al.
Nuclear medicine and biology, 43(12), 781-787 (2016-10-04)
Bone marrow is the soft tissue compartment inside the bones made up of hematopoietic cells, adipocytes, stromal cells, phagocytic cells, stem cells, and sinusoids. While [18F]-FLT has been utilized to image proliferative marrow, to date, there are no reports of
Mun-Teng Wong et al.
Journal of virology, 90(20), 9075-9095 (2016-08-05)
Hepatitis C virus (HCV) infection reorganizes cellular membranes to create an active viral replication site named the membranous web (MW). The role that human choline kinase-α (hCKα) plays in HCV replication remains elusive. Here, we first showed that hCKα activity

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