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L9381

Sigma-Aldrich

Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)

FITC conjugate, lyophilized powder

Synonyme(s) :

Bandeirea simplicifolia agglutinin, BS-I

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

Bandieraea simplicifolia

Niveau de qualité

200

Conjugué

FITC conjugate

Forme

lyophilized powder

Puissance

<100 μg per mL agglutination activity (using human blood group A erythrocytes)

Composition

Protein, ~10% Lowry

Ampleur du marquage

1-3 mol FITC per mol protein

Température de stockage

−20°C

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Actions biochimiques/physiologiques

BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Autres remarques

BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.

Forme physique

Contains phosphate buffer salts and sodium chloride

Remarque sur l'analyse

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Francesco Di Maggio et al.
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Brian B Graham et al.
American journal of respiratory and critical care medicine, 196(8), 1075-1077 (2017-03-10)
Ivanka Dimova et al.
Angiogenesis, 16(4), 921-937 (2013-07-25)
Notch is an intercellular signaling pathway related mainly to sprouting neo-angiogenesis. The objective of our study was to evaluate the angiogenic mechanisms involved in the vascular augmentation (sprouting/intussusception) after Notch inhibition within perfused vascular beds using the chick area vasculosa
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