L5037
Leptin from rat
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder
Synonyme(s) :
OB
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About This Item
Produits recommandés
Source biologique
Escherichia coli
Niveau de qualité
Produit recombinant
expressed in E. coli
Stérilité
non-sterile
Essai
≥97% (SDS-PAGE)
Forme
lyophilized powder
Puissance
≤0.8 ng/mL ED50
Poids mol.
16 kDa
Impuretés
≤1.0 EU/μg endotoxin
Numéro d'accès UniProt
Conditions d'expédition
ambient
Température de stockage
−20°C
Informations sur le gène
rat ... Lep(25608)
Application
Leptin from rat has been used:
- to check the effect of intranasal nerve growth factor (NGF) on NGF activity in the spinal cord of Sprague-Dawley rats
- for validation of labelling for leptin-bearing cells of Sprague-Dawley rats
- to study the effect of leptin on STAT3 (signal transducer and activator of transcription 3) phosphorylation in neural population of Sprague-Dawley rats
- to study leptin response towards chemoreflex in nucleus of the solitary tract
- in rats, to check the effect of association between leptin and neuronal nitric oxide pathway on penicillin-induced epileptiform activity
Actions biochimiques/physiologiques
Hormone produced primarily in adipocytes; primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight.
In rodents with uncontrolled diabetes mellitus, leptin is crucial for normalizing hyperglycemia. In the digestive tract, it also participates in the absorption of dietary molecules via controlling transporters activity.
Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.
Human and mouse leptin share ~84% sequence identity.
Human and mouse leptin share ~84% sequence identity.
Forme physique
Lyophilized from a 0.2 μm filtered solution with 4.5 mM NaHCO3.
Remarque sur l'analyse
The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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John Ciriello et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 303(7), R727-R736 (2012-08-24)
Circulating levels of leptin are elevated in individuals suffering from chronic intermittent hypoxia (CIH). Systemic and central administration of leptin elicits increases in sympathetic nervous activity (SNA), arterial pressure (AP), and heart rate (HR), and it attenuates the baroreceptor reflex
Iris A McDuffie et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 52(2), 263-273 (2004-01-20)
Leptin, the ob protein, regulates food intake and satiety and can be found in the anterior pituitary. Leptin antigens and mRNA were studied in the anterior pituitary (AP) cells of male and female rats to learn more about its regulation.
Luigi Aloe et al.
Neural regeneration research, 9(10), 1025-1030 (2014-09-11)
The purpose of this work was to investigate whether, by intranasal administration, the nerve growth factor bypasses the blood-brain barrier and turns over the spinal cord neurons and if such therapeutic approach could be of value in the treatment of
Ali Aslan et al.
Brain research, 1321, 117-124 (2010-02-02)
The aim of the present study was to investigate the role of NO as a mediator of leptin action at the penicillin-induced epileptiform activity in rat. Thirty minutes after penicillin injection, leptin, at a dose of 1 microg, significantly increased
Carmen Fanjul et al.
Journal of physiology and biochemistry, 71(2), 311-317 (2015-05-04)
Leptin is secreted into the digestive tract and contributes to the absorption of dietary molecules by regulating transporters activity. Here, we studied the effect of luminal leptin on the intestinal transport of L-glutamate, an important component of human diet. We
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