L3892
Lectine de Triticum vulgaris (blé)
peroxidase conjugate, lyophilized powder
Synonyme(s) :
Agglutinine de germe de blé, WGA
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
Triticum vulgaris
Niveau de qualité
Conjugué
peroxidase conjugate
Pureté
≥85% protein basis (Warburg-Christian)
Forme
lyophilized powder
Puissance
<40 μg/mL agglutination activity
peroxidase activity
50-200 units/mg protein
Composition
Protein, ~90% modified Warburg-Christian
Température de stockage
−20°C
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Application
Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Actions biochimiques/physiologiques
La WGA n′est pas spécifique à un groupe sanguin, mais elle présente une affinité pour les résidus N-acétyl-β-D-glucosaminyl et les oligomères du type N-acétyl-β-D-glucosamine. La WGA ne contient aucun glucide lié à une protéine.
Conditionnement
Package size based on protein content.
Définition de l'unité
One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
Forme physique
Contains citrate buffer
Notes préparatoires
Les conjugués sont préparés à partir d′une lectine purifiée par affinité.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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