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Key Documents

L3382

Sigma-Aldrich

Lectin from Helix pomatia

lyophilized powder, salt, free

Synonyme(s) :

Helix pomatia agglutinin, HPA

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Forme

lyophilized powder

Puissance

<4 μg per mL agglutination activity

Impuretés

salt, free

Température de stockage

−20°C

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Description générale

Lectin from Helix pomatia is a hexameric glycoprotein with a molecular mass of 79 kDa. It issecreted by the albumen gland of the roman snail. Helix pomatia agglutinin (HPA) is specific for N-acetylgalactosamine (GalNAc).

Application

Lectin from Helix pomatia has been used to conjugate with biotin and acridinium and used in lectin-agglutination tests.

Actions biochimiques/physiologiques

Lectin from Helix pomatia protects fertilized eggs by coating them with perivitelline fluid. They exhibit antibacterial activities and immunity in snail. Helix pomatia agglutinin (HPA) possess the potential to agglutinate pathogens. HPA has also emerged as a marker of altered Oglycosylation in cancer.
HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Notes préparatoires

Affinity purified by a modification of the method of Hammarstrom and Kabat.

Remarque sur l'analyse

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Hammarstrom, S., and Kabat, E.A.
Biochemistry, 95, 311-311 (1978)
Biochemical and structural analysis of helix pomatia agglutinin a hexameric lectin with a novel fold
Sanchez JF, et al.
The Journal of biological chemistry, 281(29), 20171-20180 (2006)
Binding of aberrant glycoproteins recognizable by Helix pomatia agglutinin in adrenal cancers
Parameswaran R, et al.
BJS open, 2(5), 353-359 (2018)
Katie Barr et al.
Transfusion, 55(1), 129-136 (2014-07-22)
The FORS blood group system (originally recognized as the Apae phenotype) was discovered by sporadic activity against polyclonal anti-A reagents and activity against the lectin Helix pomatia. The extent of monoclonal anti-A reagent activity against the FORS1 antigen is serologically
Characterization of bovine and human group B streptococci isolated in Turkey
Ekin IH and Gurturk K
Journal of Medical Microbiology, 55(5), 517-521 (2006)
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