L3160

Monoclonal Anti-Leptin antibody produced in mouse

clone LEP-13, ascites fluid

• Synonyme(s) : Anti-OB
• Numéro CAS: 93952-18-2
• Numéro MDL: MFCD00803408
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: LEP-13, monoclonal
• Poids mol.: antigen 16 kDa
• Contient: 15 mM sodium azide
• Espèces réactives: human
• Technique(s): indirect ELISA: suitable; radioimmunoassay: suitable; western blot: 1:1,000 using recombinant human leptin
• Isotype: IgG1
• Numéro d'accès UniProt: P41159
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... LEP(3952)

Description

Description générale

Leptin is a hormone secreted by adipocytes and regulates energy homeostasis, inflammation and neuroendocrine processes, synaptic formation and memory condensation. The physiological effects of leptin are mediated by leptin receptors, ObR and LepR. The receptors are present in brain, lungs, kidneys, liver and adipose tissue. Leptin is the key regulator of obesity and acts directly on neuronal networks to regulate feeding, metabolism, gastric mobility and energy homeostasis. The pathways that mediate the effect of leptin are JAK/STAT, PI3K and JNK. Deficiency of leptin may be a congenital condition and results in thyroid axis abnormalities.. Monoclonal Anti-Leptin reacts specifically with human leptin. A weak cross reactivity with mouse leptin is observed in radioimmunoassay.

Monoclonal Anti-Leptin (OB) (mouse IgG isotype) is derived from the LEP-13 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human leptin. Leptin (also known as OB, the product of the ob gene) is a 16 kDa, 146 amino acid residue, non-glycosylated polypeptide, secreted by the mature adipocyte. It is strongly expressed in white adipose tissue, and is absent or expressed at extremely low levels in other tissues. The human leptin molecule is translated as a 167 amino acid residue polypeptide, with the first 21 amino acid residues cleaved as a signal peptide.

Spécificité

The antibody reacts specifically with human leptin. By RIA, weak cross-reactivity has been observed with recombinant mouse leptin.

Immunogène

recombinant human leptin.

Application

Monoclonal Anti-Leptin antibody produced in mouse has been used in western blotting. It may be used in radioimmunoassay (RIA) and enzyme linked immunosorbent assay (ELISA).

Actions biochimiques/physiologiques

Leptin is hypothesized to be a ′′satiety factor′′, because the absence of this factor was associated with hyperphagia and obesity. Leptin is involved in appetite regulation. Human obesity is often associated with increased blood leptin levels. Leptin acts through discrete receptors and distant targets to create a feedback loop for body weight regulation. With respect to the target of leptin action, the hypothalamus has received a great deal of attention. The leptin system has also been proposed to contribute to early hematopoiesis.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable