L1006

L-Lactic Dehydrogenase from bovine heart

Type XVII, buffered aqueous glycerol solution, ≥400 units/mg protein

• Synonyme(s) : Lactate, (S)-Lactate: NAD⁺ oxidoreductase, L-LDH, LAD, LD
• Numéro CAS: 9001-60-9
• Numéro de classification (Commission des enzymes): 1.1.1.27 (BRENDA, IUBMB)
• Numéro CE : 232-617-8
• Numéro MDL: MFCD00131466
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bovine heart
• Niveau de qualité: 200
• Type: Type XVII
• Pureté: 5.0—15.0 mg protein/mL (biuret)
• Forme: buffered aqueous glycerol solution
• Activité spécifique: ≥400 units/mg protein
• Poids mol.: 140 kDa
• Fabricant/nom de marque: Sigma-Aldrich
• Technique(s): cell based assay: suitable
• Impuretés: <10 μg/mg protein free ammonium
• Couleur: white
• pH: 7.5
• Traces de cations: NH₄⁺: ≤10 μg/mg protein
• Numéro d'accès UniProt: P19858; Q5E9B1
• Application(s): life science and biopharma
• Activité étrangère: glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase ≤0.02%; pyruvate kinase, myokinase and α-glycerophosphate dehydrogenase ≤0.01%
• Température de stockage: 2-8°C
• Informations sur le gène: cow ... LDHA(281274) , LDHB(281275)

Description

Description générale

Research area: Cell signaling. L-LacticDehydrogenase (LDH) is present in the cell cytoplasm. and is part of the glycolytic pathway. Lactate dehydrogenase (LDH) is a ubiquitous molecule found in plants, yeast, mammals, and microorganisms and is a member of the oxidoreductase family. It has five isozymes (LD₁ to LD₅) and this composition varies in different tissues. LD₁ is present at higher concentrations in the heart, kidneys, and erythrocytes. LD₅ is found in the liver and skeletal muscles.(2)

Application

L-Lactic Dehydrogenase from bovine heart has been used in ATPase assay of R2 complex Rvb1p-Rvb2p, RecA protein and sarcoplasmic reticulum Ca²⁺-ATPase (SERCA).

Actions biochimiques/physiologiques

Variation in the levels of L-Lactic Dehydrogenase (LDH) isoform is a diagnostic marker for tissue damage. The levels of LDH is useful in detecting dairy cattle disease, Mastitis. Dye based purified LDH from bovine heart has analytical applications.Lactic dehydrogenase (LDH)plays an essential role in the glycolytic pathway where it is involved in the conversionof pyruvate to lactate using NAD⁺as a co-factor. It is considered an important molecule that can be used incancer therapy as it acts as a glycolytic inhibitor. Inhibition of LDH isassociated with blocking of aerobic glycolysis in the tumour cells.

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Définition de l'unité

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Forme physique

Solution in 50% glycerol containing 0.025 M potassium phosphate buffer, pH 7.5

Remarque sur l'analyse

Protein determined by biuret

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1