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K0514

Sigma-Aldrich

Anti-KCNK9 (TASK-3) antibody, Mouse monoclonal

clone KCN-75, purified from hybridoma cell culture

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

KCN-75, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~40 kDa (representing the KCNK9 monomer, and a weaker band at 80 kDa representing the KCNK9 dimer)

Espèces réactives

human

Technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 4-8 μg/mL using whole extract of cultured T-47D cells (human breast carcinoma)

Isotype

IgG2b

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... KCNK9(51305)

Catégories apparentées

Description générale

Monoclonal Anti- KCNK9 (TASK-3), Clone KCN-75, (mouse IgG2b isotype) is derived from the KCN-75 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide. Potassium two pore domain channel subfamily K member 9 (KCNK9) gene has been localized to the chromosomal region 8q24.
The gene KCNK9 (potassium two pore domain channel subfamily K member 9), also referred to as TWIK-related acid sensitive K(+) channel-3 (TASK-3), encodes a new member of the K2P (two pore domain K+ channel) family of proteins. It is found to be expressed in several rat tissues, such as brain, kidney, liver, lung, colon, stomach, spleen, testis, and skeletal muscle. It is also expressed to a lesser extent in heart and small intestine. All the potassium channels contain a conserved P domain that is involved in providing K+ selectivity and two, four, or six transmembrane segments.

Immunogène

synthetic peptide corresponding to amino acids 360-374 in the C-terminus of human KCNK9.

Application

Anti-KCNK9 (TASK-3) antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunohistochemistry
  • immunocytochemistry

Actions biochimiques/physiologiques

Potassium two pore domain channel subfamily K member 9 (KCNK9) is expressed in carcinomas including breast, lung, colon as well as in metastatic prostate. In 10% of breast cancer patients this gene is amplified, and in 44% the protein is overexpressed.
The gene KCNK9 (potassium two pore domain channel subfamily K member 9) encodes a tandem pore domain potassium channel that dimerizes to form a K+-selective pore. It is highly sensitive to change in pH and is involved in background leak potassium currents found in many cell types. The channel increases the hypoxia tolerance of malignantly transformed cells and facilitates its proliferation and/or survival. It may serve as a potential target for cancer therapies. Mutations in this gene can cause defects in the neuronal migration and have been associated with Birk Barel mental retardation dysmorphism syndrome. This oncogenic potassium channel is found to be expressed in epithelial ovarian cancer and patients with increased expression of this protein have better survival rates.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Oncogenic potential of TASK3 (Kcnk9) depends on K+ channel function
Pei L, et al.
Proceedings of the National Academy of Sciences of the USA, 100(13), 7803-7807 (2003)
TASK-5, a new member of the tandem-pore K(+) channel family.
Kim D and Gnatenco C
Biochemical and Biophysical Research Communications, 284, 923-930 (2001)
TASK-3, a novel tandem pore domain acid-sensitive K+ channel. An extracellular histiding as pH sensor.
Rajan S
The Journal of Biological Chemistry, 275, 16650-16657 (2000)
Expression and prognostic significance of the oncogenic K2P potassium channel KCNK9 (TASK-3) in ovarian carcinoma.
Innamaa A
Anticancer Research, 33, 1401-1408 (2013)
Recovery of current through mutated TASK3 potassium channels underlying Birk Barel syndrome.
Veale EL
Molecular Pharmacology, 85, 397-407 (2014)

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