I9640
IgG from bovine serum
technical grade, ≥80% (SDS-PAGE), buffered aqueous solution
Synonyme(s) :
IgG bovine
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About This Item
Produits recommandés
Conjugué
unconjugated
Niveau de qualité
Qualité
technical grade
Essai
≥80% (SDS-PAGE)
Forme
buffered aqueous solution
Technique(s)
cell culture | mammalian: suitable
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
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Description générale
Bovine immunoglobulin, protein source obtained from immunoglobulin derived from serum, is a naturally occurring bioactive proteins and peptides. Fetal calf or bovine serum IgG can be depleted with protein G affinity chromatography and various concentration of this product can be used in RPMI-1640 medium to produce mouse hybridoma cell line CAy-G (anti-HBs IgG1 mAb producing hybridoma cell). The heat aggregated bovine serum IgG is also useful in down regulating mannose receptor expression. IgG from bovine serum reacts specifically with mouse.
Immunogène
IgG is isolated from pooled bovine serum.
Application
IgG from bovine serum is used as a supplement in cell culture.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.2 with 15 mM sodium azide as preservative
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
S Schreiber et al.
Journal of immunology (Baltimore, Md. : 1950), 147(4), 1377-1382 (1991-08-15)
We previously described the presence of an inhibitory protein contained in the 20 to 40% (NH4)2SO4 precipitable fraction of FCS that down-regulates expression of mannose receptors on bone marrow-derived macrophages. We now identify aggregated bovine IgG as the main inhibitory
C Aybay et al.
Journal of immunological methods, 233(1-2), 77-81 (2000-01-29)
Fetal calf serum (FCS) was depleted of its immunoglobulin G (IgG) in a rapid procedure using protein G affinity chromatography. 20 ml of FCS was depleted of its IgG in less than 80 min by applying 5 ml of FCS
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