I7642
Anti-Interleukin-12 antibody produced in goat
IgG fraction of antiserum
Synonyme(s) :
Anti-IL-12
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Source biologique
goat
Niveau de qualité
Forme d'anticorps
IgG fraction of antiserum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
mouse
Technique(s)
neutralization: suitable
western blot: 1-2 μg/mL
Numéro d'accès UniProt
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
mouse ... Il12a(16159)
Description générale
Interleukin-12 (IL-12) is an important cytokine that links innate and adaptive immunity. It plays a role in the regulation of Th1-associated cellular immunity to foreign pathogens, and humoral immunity to antigens that are both T cell-independent and T cell-dependent. IL-12 is primarily produced by innate immune cells, dendritic cells, epithelial cells and macrophages. All forms of IL-12 bind to the IL-12R which includes IL-12RbI and IL-12RbII subunits expressed on T cells and natural cells. IL-12 stimulates the production of IFN-γ and TNF-α, subsequent production of nitric oxide that leads to pathogen destruction. Reports suggest the involvement of JAK/STAT pathway components in mediating these effects. The effects of IL-12 have been implicated in bacterial, fungal and viral infections. Due to its role in inducing matrix metalloproteases, it also has important role in the etiology of chronic B-cell malignancies and lung adenocarcinomas
Anti-Interleukin-12 antibody recognizes mouse IL-12. It shows < 10% cross-reactivity with recombinant human IL-12 and < 5% cross-reactivity with recombinant porcine IL-12.
Anti-Interleukin-12 antibody recognizes mouse IL-12. It shows < 10% cross-reactivity with recombinant human IL-12 and < 5% cross-reactivity with recombinant porcine IL-12.
Spécificité
The antibody will neutralize the biological activity of recombinant mouse IL-12. Based on ELISA, the antibody shows ~10% cross-reactivity with recombinant human IL-12 and <5% cross-reactivity with recombinant porcine IL-12.
Immunogène
purified, insect cell line Sf21-derived, recombinant mouse IL-12.
Application
Anti-Interleukin-12 antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. The antibody is also suitable for neutralization reactions. It has been used to neutralize IL-12 in the extracts of murine splenocytes.
Forme physique
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.
Notes préparatoires
Purified using protein G.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
IL-12 and Related Cytokines: Function and Regulatory Implications in Candida albicans Infection
Ashman RB et al
Clinical & Developmental Immunology, doi: 10-doi: 10 (2011)
David J Klinke
Molecular cancer, 9, 242-242 (2010-09-17)
Monoclonal antibodies represent some of the most promising molecular targeted immunotherapies. However, understanding mechanisms by which tumors evade elimination by the immune system of the host presents a significant challenge for developing effective cancer immunotherapies. The interaction of cancer cells
Dennis W Metzger
Cytokine, 52(1-2), 102-107 (2010-07-24)
Interleukin (IL)-12 is a pivotal cytokine that strongly stimulates Th1-associated cellular immunity. It is now recognized that IL-12 also activates humoral immunity to both T-dependent and T-independent antigens. This has let to considerable interest in exploiting IL-12 as a vaccine
Anne O'Garra et al.
Nature immunology, 10(9), 929-932 (2009-08-21)
The authors recount their discovery of how pathogen-induced interleukin 12 production leads to T(H)1 T cell polarization. Simultaneously they discovered the suppressive cytokine interleukin 10 inhibits antigen-presenting cells, thus regulating development of T(H)1 cells.
John L Langowski et al.
Nature, 442(7101), 461-465 (2006-05-12)
Chronic inflammation has long been associated with increased incidence of malignancy and similarities in the regulatory mechanisms have been suggested for more than a century. Infiltration of innate immune cells, elevated activities of matrix metalloproteases and increased angiogenesis and vasculature
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique