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I3019

Sigma-Aldrich

Interleukin-10 from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, suitable for cell culture

Synonyme(s) :

IL-10

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About This Item

Numéro MDL:
MFCD00212207
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

mouse

Niveau de qualité

200

Produit recombinant

expressed in E. coli

Pureté

>97% (SDS-PAGE)

Forme

lyophilized powder

Puissance

0.01-1.50 ng/mL EC50

Poids mol.

~18 kDa

Conditionnement

pkg of 5 μg

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Numéro d'accès UniProt

P18893

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

mouse ... Il10(16153)

Application

Interleukin-10 from mouse has been used as a standard in ELISA (enzyme-linked immunosorbent assay) cytokine assay.

Actions biochimiques/physiologiques

Interleukin-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.
Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.

Forme physique

Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein

Remarque sur l'analyse

The biological activity of IL-10 is determined in a cell proliferation assay using MC/9 cells.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Go Kuwahara et al.
PloS one, 9(9), e106987-e106987 (2014-09-10)
Novel cell therapy is required to treat critical limb ischemia (CLI) as many current approaches require repeated aspiration of bone marrow cells (BMCs). The use of cultured BMCs can reduce the total number of injections required and were shown to
Heather M McGee et al.
Immunology and cell biology, 89(7), 767-776 (2011-01-19)
Neonates have a developing immune response, with a predisposition towards induction of tolerance. As the immune system develops, immunity rather than tolerance is induced, with this development of immunity occurring in response to external factors such as the environment. As
Sergio M Borghi et al.
Experimental physiology, 100(5), 531-544 (2015-02-26)
What is the central question of this study? This study investigated the role of the endogenous anti-inflammatory cytokine interleukin-10 in intense acute swimming-induced muscle mechanical hyperalgesia in mice. What is the main finding and its importance? Endogenous interleukin-10 has a
Jui-Hung Yen et al.
Journal of leukocyte biology, 98(5), 689-702 (2015-06-11)
MS is an autoimmune disease characterized by immune cell infiltration in the CNS, leading to cumulative disability. IFN-β, used clinically in RR-MS reduces lesion formation and rates of relapse. Although the molecular mechanisms are not entirely elucidated, myeloid cells appear
Philipp Lichte et al.
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft, 200, 73-78 (2015-03-25)
Musculoskeletal injuries induce systemic inflammation which often impairs lung function contributing to morbidity. IL-10 has been shown to have a beneficial effect on immune dysfunction and organ damage after different traumatic insults. We sought to investigate the effect of inhalative
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