I0523

Interleukin-2 from mouse

IL-2, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonyme(s) : mIL-2, IL-2, T-cell growth factor
• Numéro MDL: MFCD00164816
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Produit recombinant: expressed in E. coli
• Pureté: ≥97% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: ≤0.2 ng/mL ED₅₀
• Qualité: endotoxin tested
• Poids mol.: 17.2 kDa
• Conditionnement: pkg of 5X20 μg; pkg of 20 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤1.000 EU/μg
• Couleur: white
• Numéro d'accès UniProt: P04351
• Température de stockage: −20°C
• Informations sur le gène: mouse ... Il2(16183)

Description

Description générale

IL-2 (interleukin-2) codes for a pro-inflammatory cytokine. IL-2 is mostly expressed in T helper cells.

Application

Interleukin-2 from mouse has been used to induce sepsis in rat model. It has also been used in dendritic epidermal T cell culture.

Actions biochimiques/physiologiques

Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lymphokine promotes long-term growth of activated T cells and related cell types. IL-2 plays a role in the activation and proliferation of natural killer cells, induces γ-interferon and B cell growth factor secretion, and modulates the expression of the IL-2 receptor. IL-2 expression is known to reduce with age and results in influenza infection, neoplasms and lethal autoimmunity. IL-2 participates in T cell proliferation and differentiation and controls the homeostasis of T cell. As a part of immune response, IL-2 stimulates the action of T helper cell 1 and 2 along with endothelial white blood cells of skeletal muscles. IL-2 regulation is controlled by c-rel protein. IL-2 is a neurotrophic molecule and has special functions in cortical, hippocampal, septal, cerebellar and retinal neurons.

Forme physique

Lyophilized from a 0.2 μm filtered solution in 10 mM sodium citrate, pH 4.0.

Remarque sur l'analyse

Activity was determined by dose-dependent stimulation of murine CTLL-2 cells. ED₅₀ : ≤ 0.2 ng/ml (corresponds to a specific activity of 5 × 10⁶ units/mg)

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)