H8032

Hanahan′s Broth (SOB Medium)

powder microbial growth medium

• Synonyme(s) : Super Optimal Broth
• Code UNSPSC : 41106200
• Nomenclature NACRES : NA.85

Propriétés

• Qualité: for molecular biology
• Niveau de qualité: 100
• Description: recommended for cultivation of recombinant E. coli.
• Stérilité: non-sterile
• Forme: powder
• Composition: KCl, 0.186 g/L ; MgSO₄, 2.4 g/L ; NaCl, 0.5 g/L ; Tryptone, 20 g/L ; Yeast extract, 5 g/L
• Technique(s): microbiological culture: suitable
• pH: 6.8-7.2(2.8% solution)
• Application(s): food and beverages; microbiology
• Température de stockage: room temp
• Adéquation: nonselective for Escherichia coli; nonselective for coliforms

Description

Description générale

Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). Casein enzymic hydrolysate and yeast extract supply nitrogenous compounds and growth factors for recombinant Escherichia coli. Potassium chloride and sodium chloride maintain the isotonic condition. Magnesium sulfate is added to the medium as a necessary component for DNA replication.

Application

Hanahan′s Broth is recommended for use in cultivation of recombinant strains of Escherichia coli. It is suitable for the preparation of competent E. coli., often with the addition of 0.4% dextrose. It has also been used for the preparation of C. necator H16 competent cells. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.

Caractéristiques et avantages

Hannahan′s Broth (SOB) powder provides:

• Standard formulation
• An inexpensive alternative to liquid

Reconstitution

Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Autres remarques

Casein enzymic hydrolysate and yeast extract supply the necessary nitrogenous compounds and growth factors. Potassium chloride and sodium chloride provide an isotonic environment for the cells. Magnesium sulfate aids DNA replication.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves