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Key Documents

H4149

Sigma-Aldrich

Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse

clone LK1, ascites fluid

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

LK1, monoclonal

Poids mol.

antigen 60 kDa

Contient

15 mM sodium azide

Espèces réactives

rat, human, chicken

Technique(s)

electron microscopy: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
western blot: 1:400 using cultured human foreskin fibroblast extract

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... HSPD1(3329)
rat ... Hspd1(63868)

Description générale

Heat shock (stress) proteins (HSP) are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. HSP-60 is a member of HSP family with a uniquely high level of sequence conservation.
Monoclonal Anti-Heat Shock Protein 60 (mouse IgG1 isotype) is derived from the LK1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human heat diseases.

Spécificité

The antibody recognizes an epitope located between amino acid residues 383-447 of the human HSP 60. It is not cross-reactive with the bacterial counterpart (E. coli), or with helminth and spinach HSP60. The antibody reacts with constitutive and inducible HSP60. It demonstrates a raised level of staining in immunohistochemistry of synovial membranes taken from patients with juvenile chronic arthritis.

Immunogène

recombinant human heat shock protein 60 (HSP60)

Application

Monoclonal Anti-Heat Shock Protein 60 has been used in the following techniques:
  • Enzyme linked immunosorbent assay (ELISA)
  • Immunoblotting
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoelectron microscopy
  • Double immunofluorescence

Actions biochimiques/physiologiques

A wide variety of environmental perturbations, such as a sudden increase in temperature, induce cells to rapidly synthesize a group of polypeptides known as the heat shock (stress) proteins. HSP-60 is considered to be a potential antigen in a number of autoimmune diseases. Abnormal immune reactivity involving shock protein 60 (HSP60) has also been implicated in the pathogenesis of schizophrenia. This monoclonal antibody enables the differentiation between the HSP60 of mammalian and bacterial origin.
Abnormal immune reactivity involving HSP60 has also been implicated in the pathogenesis of schizophrenia.

Forme physique

Monoclonal Anti-Heat Shock Protein 60 is provided as ascites fluid with 0.1% sodium azide as a preservative.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Studies characterizing 60 kda autoantibodies in subjects with schizophrenia
Wang X, et al.
Biological Psychiatry, 53(5), 361-375 (2003)
HSP60: issues and insights on its therapeutic use as an immunoregulatory agent
Coelho V and Faria A
Frontiers in Immunology, 2, 97-97 (2012)
Claudia Campanella et al.
Oncotarget, 7(20), 28849-28867 (2015-12-25)
HSP60 undergoes changes in quantity and distribution in some types of tumors suggesting a participation of the chaperonin in the mechanism of transformation and cancer progression. Suberoylanilide hydroxamic acid (SAHA), a member of a family of histone deacetylase inhibitors (HDACi)
Hsp60 and Hsp10 increase in colon mucosa of Crohn?s disease and ulcerative colitis
Rodolico V, et al.
Cell Stress & Chaperones, 15(6), 877-884 (2010)
F Rappa et al.
European journal of histochemistry : EJH, 57(2), e20-e20 (2013-06-29)
In this work we analysed, by immunohistochemistry, a series of brain tumors to detect the levels and cellular distribution of Hsp60 and Hsp70. We found that Hsp60 levels were significantly higher than those of Hsp70 in neuroepithelial tumors, while levels

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