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GERPN2134

ECL™ Western Blotting Reagents

Cytiva RPN2134

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About This Item

Code UNSPSC :
41105332
Nomenclature NACRES :
NA.32

Caractéristiques

wetted part: no

Conditionnement

pkg of 1 kit

Fabricant/nom de marque

Cytiva RPN2134

Couleur

Detection reagent 1: Colorless Detection reagent 2: Colorless

Température de stockage

2-8°C

Catégories apparentées

Description générale

Amersham™ ECL™ Western Blotting Detection Reagents were the world′s first commercial chemiluminescent reagents for Western blotting and are still the most widely used. They are at least 10-fold more sensitive than colorimetric assays and produce excellent and reliable results every time.

These reagents use enhanced luminol-based detection which is suitable for all routine western blotting. ECL™ Western Blotting Detection Reagents in combination with Hybond® ECL™ membranes form a complete solution for Western blot applications.

Contents include the following:
GERPN2109, 62.5 mL each of ECL™ Detection Reagents 1 and 2 (1000 cm2)
GERPN2209, 125 mL each (2000 cm2)
GERPN2106, 250 mL each (4000 cm2)
GERPN2134, 375 mL each (6000 cm2)

Application

ECL™ Western Blotting Reagents have been used in Western blotting.

Caractéristiques et avantages

  • High sensitivity, ~10 pg in model system.
  • Proven performance, the most frequently cited ECL™ Western Blotting Detection Reagents in publications
  • Detectable on Hyperfilm ECL™ or with CCD imager
  • Recommended membrane Hybond ECL™

Composants

The kit contains
3 x 125 mL of ECL™ Detection reagent 1
3 x 125 mL of ECL™ detection reagent 2

Sufficient for detection of 6000 cm2 of membrane:
375 mL Detection reagent 1(color : colorless)
375 mL Detection reagent 2 (color: colorless)

Stockage et stabilité

Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

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Sun CY, et al.
Molecular Medicine Reports, 13(6), 5349-5357 (2016)

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Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

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Protocoles

Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from GE Healthcare.

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This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using GE Healthcare products.

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