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GE28-9909-45

Sigma-Aldrich

Superdex® 200 Increase 5/150 GL

Cytiva 28-9909-45, column L × I.D. 15 cm × 5 mm, 8.6 μm particle size

Synonyme(s) :

Size-exclusion chromatography column

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About This Item

Code UNSPSC :
41115700
Nomenclature NACRES :
SB.52

Matériaux

glass column
polyethylene filter

Gamme de produits

Superdex®

Poids mol.

10000-600000 Da (globular proteins)

Fabricant/nom de marque

Cytiva 28-9909-45

Conditionnement

1 ea of

Paramètres

<0.75 mL/min flow rate
<0.75 ml/min flow rate
<3.0 Mpa (725 psi)
4-50 μL sample volume

Volume du lit

~3 mL

Colonne, L × D.I.

15 cm × 5 mm

Matrice

agarose dextran composite
polymeric

Groupe de la matrice active

phenyl phase

Taille des particules

8.6 μm

cleaning

1-14

working range

3-12

Technique de séparation

size exclusion (SEC)

Catégories apparentées

Description générale

Superdex 200 is a column for rapid purity check and screening by high resolution gel filtration for proteins with molecular weights between 10 000 and 600 000, such as antibodies. Pre-packed with small rigid agarose beads for high flow rates and pH stability.

Adéquation

Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride
Well suited for rapid purity checks and quick screenings of experimental conditions.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Produits recommandés

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Informations légales

Superdex is a registered trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Performing a purity and homogeneity check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from GE Healthcare.

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Protocoles

Performing a Separation with Superdex

Superdex from GE Healthcare are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Refolding using ion exchange chromatography and analysis of refolding

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.

Solubilization of Membrane Proteins

This page shows how to solubilize membrane proteins with products from GE Healthcare.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

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