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Key Documents

GE27-4577-01

Sigma-Aldrich

Anti-GST Antibody

Cytiva 27-4577-01

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About This Item

Code UNSPSC :
12352116
Nomenclature NACRES :
NA.32

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Fabricant/nom de marque

Cytiva 27-4577-01

Conditionnement

pkg of 0.5 mL, 50 detections

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Catégories apparentées

Description générale

Anti-GST Antibody is a polyclonal antibody purified from the sera of goats immunized with purified schistosomal GST. Because of its polyclonal nature, it can recognize more than one epitope on GST, thereby improving its capacity for recognizing GST fusion proteins even if some binding sites are masked due to recombinant protein folding.

Application

Western Blots

Caractéristiques et avantages

  • For the highly sensitive and specific detection of recombinant schistosomal GST fusion proteins expressed using pGEX vectors
  • Extensively cross-adsorbed to remove antibodies that bind to E. coli proteins and affinity purified using Glutathione Sepharose™
  • Supplied unconjugated for use with any enzyme-conjugated anti-goat antibody
  • Recommended for use in Western blots and dot blots to detect GST fusion proteins

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

GST Gene Fusion Vectors
A license for commercial use of GST Gene Fusion Vectors under US patent 5,654,176 and equivalent patents and patent applications in other countries must be obtained from Millipore Corp (formerly Chemicon International Inc).
Sepharose is a trademark of Cytiva

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Code de la classe de stockage

12 - Non Combustible Liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Claire E J Moore et al.
Cellular signalling, 28(4), 284-293 (2016-01-23)
Eukaryotic elongation factor 2 kinase (eEF2K) inhibits the elongation stage of protein synthesis by phosphorylating its only known substrate, eEF2. eEF2K is tightly regulated by nutrient-sensitive signalling pathways. For example, it is inhibited by signalling through mammalian target of rapamycin
Apoorva Baluapuri et al.
Molecular cell, 74(4), 674-687 (2019-04-01)
The MYC oncoprotein binds to promoter-proximal regions of virtually all transcribed genes and enhances RNA polymerase II (Pol II) function, but its precise mode of action is poorly understood. Using mass spectrometry of both MYC and Pol II complexes, we

Articles

Troubleshooting of Detection Methods of GST-tagged Proteins

Troubleshooting of Detection Methods of GST-tagged Proteins

Protocoles

Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from GE Healthcare.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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