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Key Documents

GE17-6000-87

IPG-Buffer, pH 3-10

Cytiva 17-6000-87

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About This Item

Code UNSPSC :
41105323
Nomenclature NACRES :
NA.25

Stérilité

non-sterile

Forme

liquid

Caractéristiques

wetted part: no

Conditionnement

pkg of 1 mL

Fabricant/nom de marque

Cytiva 17-6000-87

Couleur

Colorless to light yellow

Plage de pH

3-10

Solubilité

cold water: soluble (Soluble in cold water and hot water)

Température de stockage

2-8°C

Description générale

IPG Buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline® DryStrip gels.

Application

Each IPG Buffer type produces more uniform conductivity along the Immobiline® DryStrip during focusing, resulting in wider latitude in run times. IPG Buffers also eliminate high background staining.

IPG buffers are supplied in 1-ml aliquots of water. The buffer is diluted 50- or 200-fold in the rehydration solution, depending on the first-dimension system and pH range of the strip.

Caractéristiques et avantages

  • For use with Immobiline® DryStrip gels to improve Protein solubility.
  • Using Immobiline® DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting Low abundance Proteins.
  • Improved IPG buffer pH 3-10 and pH 3-10 NL offer improved resolution, sensitivity, and increased spot intensity as well as additional Protein spots in high molecular weight and basic pH areas.
  • Eliminates background staining.
  • Ensures optimal conditions for IEF separation.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Immobiline is a registered trademark of Cytiva

Code de la classe de stockage

12 - Non Combustible Liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Components of Rehydration Solution

This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.

Troubleshooting Second-Dimension SDS-PAGE: Multiphor™ II Electrophoresis

This page describes troubleshooting second-dimension SDS-PAGE with Multiphor™ II Electrophoresis System.

Troubleshooting First-Dimension IEF: MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit

This page describes troubleshooting first-dimension isoelectric focusing using MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit.

Protocoles

Protease Inhibition using Protease Inhibitor Mix

Protease Inhibitor Mix from GE Healthcare inhibits serine, cysteine, metalloproteases, and calpain proteases and is suitable for animal and plant tissues, yeast, and bacteria.

Processing Small Tissue or Cell Samples using Sample Grinding Kit

Sample Grinding Kit from GE Healthcare is designed to disrupt cell or tissue samples for protein extraction.

Rehydration Solutions

Rehydration Solutions

Two-Dimensional Separation of Protein Samples

This page describes two-dimensional separation of protein samples with GE Healthcare products.

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