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Key Documents

GE17-1151-01

HiTrap® Sp High Performance

Cytiva 17-1151-01, pack of 5 × 1 mL

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About This Item

Code UNSPSC :
23151816
Nomenclature NACRES :
NA.56

ligand

sulphopropyl

Description

Ion Exchanger Type (value)

Conditionnement

pack of 5 × 1 mL

Fabricant/nom de marque

Cytiva 17-1151-01

Paramètres

flow rate
42 psi

Taille du lit

7 mm × 25 mm

Volume du lit

1 mL

D.I. de la colonne

7 mm

Matrice

6% cross-linked agarose

Taille des particules

24-44 μm

Taille moy. des particules

34 μm

cleaning

3-14

working range

4-13

Adéquation

suitable for bioprocess medium

Description générale

HiTrap® SP HP are prepacked, ready-to-use SP Sepharose™ High Performance strong cation exchange columns for high-resolution, small-scale protein purification.

The small particle size (34μm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose™ High Performance is a strong cation exchange medium and Q Sepharose™ High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.

Caractéristiques et avantages

  • 34 μm bead size for high-performance, high-resolution purifications.
  • Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
  • Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
  • Strong sulfopropyl (SP) cation exchanger.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

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Protocoles

Refolding using ion exchange chromatography and analysis of refolding

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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