GE17-0440-03

Con-A Sepharose^™ 4B

Cytiva 17-0440-03, pack of 5 mL

• Code UNSPSC : 41106500
• Nomenclature NACRES : NA.56

Propriétés

• ligand: concanavalin A (con A)
• Conditionnement: pack of 5 mL
• Fabricant/nom de marque: Cytiva 17-0440-03
• Conditions de stockage: (0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)
• Matrice: 4% agarose
• Taille des particules: 45-165 μm
• Diamètre moyen: 90 μm
• cleaning in place: 4-9
• working range: 4-6
• Température de stockage: 2-8°C

Description

Description générale

Con A Sepharose^™ 4B with immobilized Concanavalin A is an affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for α-D-glucose than α-D-mannose.

Application

Con A Sepharose^™ is Concanavalin A coupled to Sepharose^™ 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloProtein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose^™ is routinely used for separation and purification of glycoProteins, polysaccharides and glycolipids

Caractéristiques et avantages

• Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
• Group-specific adsorbent for molecules containing sugars.
• Highly suitable for isolation of cell surface glycoProteins from detergent-solubilized membranes
• Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
• Highly suitable for isolation of cell surface glycoProteins from detergent-solubilized membranes
• Con A coupled to Sepharose^™ 4B via cyanogen bromide activation

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids